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GLI1 Antibody (MA5-32553) in ICC/IF
Immunocytochemistry analysis of GLI1 with HeLa cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp), incubated with recombinant monoclonal GLI1 (Product # MA5-32553) at a dilution of 1:100 (in 1% BSA in PBST overnight at 4°C), followed by Goat Anti-Rabbit IgG H&L 488 at 1:1,000 dilution (PBS used in control). Nuclear DNA was labelled in blue with DAPI, Beta tubulin (red) at 1/100 dilution (ove... View More
产品信息
MA5-32553
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JF09-08
抗原
Synthetic peptide within Human Gli1 aa 38-82
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2809830
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: GLI-Kruppel family member GLI1; Glioma-associated oncogene; glioma-associated oncogene 1; Glioma-associated oncogene homolog; glioma-associated oncogene homolog 1 (zinc finger protein); Oncogene GLI; zinc finger protein 5; Zinc finger protein GLI1
基因别名: AV235269; GLI; GLI1; Zfp-5; Zfp5
UniProt ID: (Human) P08151, (Mouse) P47806
Entrez Gene ID: (Human) 2735, (Rat) 140589, (Mouse) 14632
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
DNA binding
chromatin binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
microtubule binding
transcription regulatory region DNA binding
metal ion binding
molecular_function
nucleic acid binding
DNA-binding transcription factor
osteoblast differentiation
transcription, DNA-templated
smoothened signaling pathway
spermatogenesis
ventral midline development
positive regulation of cell proliferation
regulation of smoothened signaling pathway
response to wounding
epidermal cell differentiation
dorsal/ventral pattern formation
proximal/distal pattern formation
cerebellar cortex morphogenesis
smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation
pituitary gland development
lung development
regulation of osteoblast differentiation
positive regulation of DNA replication
positive regulation of smoothened signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
digestive tract morphogenesis
notochord regression
canonical Wnt signaling pathway
negative regulation of canonical Wnt signaling pathway
liver regeneration
regulation of hepatocyte proliferation
transcription from RNA polymerase II promoter
biological_process
regulation of transcription, DNA-templated
signal transduction
multicellular organism development
cell differentiation
