OriGene

GLI2 Monoclonal Antibody (OTI1B2), TrueMAB™

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GLI2 Antibody (CF804556) in WB

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI2 (RC217291, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI2. Positive lysates LY417413 (100 µg) and LC417413 (20 µg) can be purchased separately from OriGene.

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产品信息

CF804556

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OTI1B2

抗原

Human recombinant protein fragment corresponding to amino acids 729-1123 of human GLI2 produced in E.coli.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 8% trehalose

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

靶标信息

Gli-2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: GLI family zinc finger protein 2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; oncogene GLI2; Tax helper protein; tax helper protein 1; tax helper protein 2; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; Zinc finger protein GLI2

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基因别名: CJS; GLI2; HPE9; PHS2; THP; THP1; THP2

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UniProt ID: (Human) Q15591

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Entrez Gene ID: (Human) 2736

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding sequence-specific DNA binding DNA-binding transcription factor

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