Invitrogen

GLT-1 Recombinant Superclonal Antibody (9 HCLC)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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2 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

GLT-1 Antibody in Immunocytochemistry (ICC/IF)

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GLT-1 Antibody (711020) in ICC/IF

Immunofluorescence was performed on fixed and permeabilized U-87 MG cells for detection of endogenous EAAT2 using Anti-EAAT2 Recombinant Rabbit Polyclonal Antibody (Product # 711020, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of EAAT2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S369... View More

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GLT-1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells owing to their inherent genetic constitution. Relative expression of GLT-1 was observed in Mouse and Rat Brain and Cerebellum and not in Kidney and Lung tissues using GLT-1 Polyclonal Antibody (Product # 711020) in western blot. {RE}

产品信息

711020

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫细胞化学 (ICC/IF)

2 µg/mL

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Superclonal

类型

Antibody

克隆号

9 HCLC

抗原

Peptides corresponding to Human SLC1A2 (aa 5-20, 148-166)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2633106

产品详细信息

This antibody is predicted to react with Monkey, Sheep and Cat.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

靶标信息

Excitatory amino acid transporter 2 (EAAT2) is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis (ALS.) Alternatively spliced transcript variants of this gene have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Semaphorin 4D is upregulated in neurons of diseased brains and triggers astrocyte reactivity.

Journal of neuroinflammation

Evans EE,Mishra V,Mallow C,Gersz EM,Balch L,Howell A,Reilly C,Smith ES,Fisher TL,Zauderer M

711020 was used in Immunocytochemistry-immunoflourescence to suggest that SEMA4D blockade may ameliorate disease pathology by preserving normal astrocyte function and reducing the negative consequences of reactive astrogliosis.

Sat Aug 06 00:00:00 EDT 2022

Semaphorin 4D is upregulated in neurons of diseased brains and triggers astrocyte reactivity.

Journal of neuroinflammation

Evans EE,Mishra V,Mallow C,Gersz EM,Balch L,Howell A,Reilly C,Smith ES,Fisher TL,Zauderer M

Sat Aug 06 00:00:00 EDT 2022

ALS-associated genes in SCA2 mouse spinal cord transcriptomes.

Human molecular genetics

Scoles DR,Dansithong W,Pflieger LT,Paul S,Gandelman M,Figueroa KP,Rigo F,Bennett CF,Pulst SM

711020 was used in Western Blotting to investigate the relationship of ATXN2 with motor neuron dysfunction in vivo.

Sat Jun 27 00:00:00 EDT 2020

生物信息学

蛋白别名: EAAT2 variant mEAAT2/5UT5; Excitatory amino acid transporter 2; excitotoxic amino acid transporter 2; glial high affinity glutamate transporter; GLT-1; GLT1a; GLT1b; GLT1c; GLUT-R; glutamate transporter 1c; Glutamate/aspartate transporter II; high affinity glucose transporter; OTTHUMP00000165209; OTTHUMP00000165210; Sodium-dependent glutamate/aspartate transporter 2; solute carrier family 1 (glial high affinity glutamate transporter), member 2; Solute carrier family 1 member 2; solute carrier family 1, member 2

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基因别名: 1700091C19Rik; 2900019G14Rik; AI159670; EAAT2; Glt; GLT-1; GLT1; HBGT; MGLT1; SLC1A2

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UniProt ID: (Human) P43004, (Mouse) P43006, (Rat) P31596

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Entrez Gene ID: (Human) 6506, (Mouse) 20511, (Rat) 29482

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功能

L-glutamate transmembrane transporter activity high-affinity glutamate transmembrane transporter activity protein binding amino acid transmembrane transporter activity glutamate:sodium symporter activity symporter activity sodium:dicarboxylate symporter activity primary active transporter

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