Target of Interest
Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Application
图: 1 / 4
GLUT3 Antibody (MA5-32697) in ICC/IF
Immunofluorescence analysis of GLUT3 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GLUT3 Rabbit Recombinant Monoclonal Antibody (JA50-31) (Product # MA5-32697) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Pro... View More
产品信息
MA5-32697
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JA50-31
抗原
Synthetic peptide within Human GLUT3 aa 11-60
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2809974
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF1 induced signaling. GLUT3 is detectable in a few normal cell type spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. GLUT3 staining is also detectable in human cancers including those of the ovary, lung, and testis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: FLJ90380; glucose transporter 3; Glucose transporter type 3, brain; GLUT-3; solute carrier family 2 (facilitated glucose transporter), member 3; Solute carrier family 2 A3 (neuron glucose transporter); Solute carrier family 2, facilitated glucose transporter member 3; solute carrier family 2, member 2; solute carrier family 2, member 3
基因别名: AA408729; AL023014; AL024341; AU040424; C78366; Glut-3; GLUT3; SLC2A3
UniProt ID: (Human) Q9UG15, (Mouse) P32037, (Rat) Q07647
Entrez Gene ID: (Human) 6515, (Mouse) 20527, (Rat) 25551
glucose transmembrane transporter activity
glucose binding
dehydroascorbic acid transporter activity
D-glucose transmembrane transporter activity
transporter activity
sugar:proton symporter activity
monosaccharide transmembrane transporter activity
galactoside binding
kinase binding
transmembrane transporter activity
substrate-specific transmembrane transporter activity
xylose binding
protein binding
transport
carbohydrate transport
glucose transport
proton transport
hexose transmembrane transport
glucose import
transmembrane transport
dehydroascorbic acid transport
cation transmembrane transport
carbohydrate metabolic process
L-ascorbic acid metabolic process
glucose transmembrane transport
response to glucose
monosaccharide transport
negative regulation of apoptotic process
