Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 1
GM-CSF Antibody (MA5-44948) in WB
Western blot analysis of GM-CSF in recombinant protein (50 ng/Lane). Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in GM-CSF Monoclonal antibody (Product # MA5-44948) using a dilution of 1:500 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Mouse IgG - HRP secondary antibody at a dilution of 1:100,000 for 1 hour at room temperature. Predicted band size: 25 kDa. Observed band size: 25 kDa.
产品信息
MA5-44948
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
A6C6
抗原
Recombinant protein within Human GM-CSF aa 1-144/144.
偶联物
形式
Liquid
浓度
2 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931404
产品详细信息
Application notes: Indirect ELISA
靶标信息
The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; GM-CSF; granulocyte macrophage colony stimulating factor; granulocyte macrophage-colony stimulating factor; Granulocyte-macrophage colony-stimulating factor; M-GM-CSF; MGC131935; MGC138897; MGC151255; MGC151257; Molgramostin; Sargramostim
基因别名: CSF2; GMCSF
UniProt ID: (Human) P04141
Entrez Gene ID: (Human) 1437
MAPK cascade
embryonic placenta development
immune response
positive regulation of cell proliferation
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
peptidyl-tyrosine phosphorylation
positive regulation of interleukin-23 production
epithelial fluid transport
macrophage activation
positive regulation of tyrosine phosphorylation of Stat5 protein
myeloid dendritic cell differentiation
positive regulation of GTPase activity
positive regulation of DNA replication
negative regulation of cytolysis
cellular response to lipopolysaccharide
positive regulation of podosome assembly
dendritic cell differentiation
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
