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Proteintech

GPD2 Polyclonal Antibody

12 篇参考文献

查看其他10个GPD2抗体

说明书

实验方案

技术支持

货号 17219-1-AP

此产品在中国无法购买

150 µL

说明书

实验方案

技术支持

抗体检测数据 (12)

Application

GPD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 12

GPD2 Antibody (17219-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using (Product # 17219-1-AP) (GPD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPD2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

产品信息

17219-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:4,000

查看11篇已发表文章 11篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫沉淀 (IP)

0.5-4.0 µg

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

GPD2 Fusion Protein Ag11036 (5-378 aa encoded by BC019874)

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

0.13 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

Immunogen sequence: KAVKGTLVG GGALATVLGL SQFAHYRRKQ MNLAYVKAAD CISEPVNREP PSREAQLLTL QNTSEFDILV IGGGATGSGC ALDAVTRGLK TALVERDDFS SGTSSRSTKL IHGGVRYLQK AIMKLDIEQY RMVKEALHER ANLLEIAPHL SAPLPIMLPV YKWWQLPYYW VGIKLYDLVA GSNCLKSSYV LSKSRALEHF PMLQKDKLVG AIVYYDGQHN DARMNLAIAL TAARYGAATA NYMEVVSLLK KTDPQTGKVH VSGARCKDVL TGQEFDVRAK CVINATGPFT DSVRKMDDKD AAAICQPSAG VHIVMPGYYS PESMGLLDPA TSDGRVIFFL PWQKMTIAGT TDTPTDVTHH PIPSEEDINF ILNEV (5-378 aa encoded by BC019874)

靶标信息

Mitochondrial glycerophosphate dehydrogenase (EC 1. 1. 99. 5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Uncoupled glycerol-3-phosphate shuttle in kidney cancer reveals that cytosolic GPD is essential to support lipid synthesis.

Molecular cell

Yao CH,Park JS,Kurmi K,Hu SH,Notarangelo G,Crowley J,Jacobson H,Hui S,Sharpe AH,Haigis MC

Thu Apr 20 00:00:00 UTC 2023

Mitochondrial Glycerol-3-Phosphate Dehydrogenase Restricts HBV Replication via the TRIM28-Mediated Degradation of HBx.

Journal of virology

Liu C,Zhao K,Chen Y,Yao Y,Tang J,Wang J,Xu C,Yang Q,Zheng Y,Yuan Y,Sun H,Zhang Y,Zhou Y,Chen J,Wang Y,Wu C,Pei R,Chen X

Wed May 31 00:00:00 UTC 2023

Mitochondrial Glycerol-3-Phosphate Dehydrogenase Restricts HBV Replication via the TRIM28-Mediated Degradation of HBx.

Journal of virology

Liu C,Zhao K,Chen Y,Yao Y,Tang J,Wang J,Xu C,Yang Q,Zheng Y,Yuan Y,Sun H,Zhang Y,Zhou Y,Chen J,Wang Y,Wu C,Pei R,Chen X

Wed May 31 00:00:00 UTC 2023

A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria.

Proceedings of the National Academy of Sciences of the United States of America

Wu S,Mao C,Kondiparthi L,Poyurovsky MV,Olszewski K,Gan B

Tue Jun 28 00:00:00 UTC 2022

Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD⁺ to alleviate mitochondrial disease.

Cell metabolism

Liu S,Fu S,Wang G,Cao Y,Li L,Li X,Yang J,Li N,Shan Y,Cao Y,Ma Y,Dong M,Liu Q,Jiang H

Tue Oct 05 00:00:00 UTC 2021

Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.

eLife

Le Vasseur M,Friedman J,Jost M,Xu J,Yamada J,Kampmann M,Horlbeck MA,Salemi MR,Phinney BS,Weissman JS,Nunnari J

Wed May 26 00:00:00 UTC 2021

Low metformin causes a more oxidized mitochondrial NADH/NAD redox state in hepatocytes and inhibits gluconeogenesis by a redox-independent mechanism.

The Journal of biological chemistry

Alshawi A,Agius L

Fri Feb 22 00:00:00 UTC 2019

Low metformin causes a more oxidized mitochondrial NADH/NAD redox state in hepatocytes and inhibits gluconeogenesis by a redox-independent mechanism.

The Journal of biological chemistry

Alshawi A,Agius L

Fri Feb 22 00:00:00 UTC 2019

Glycerol phosphate shuttle enzyme GPD2 regulates macrophage inflammatory responses.

Nature immunology

Langston PK,Nambu A,Jung J,Shibata M,Aksoylar HI,Lei J,Xu P,Doan MT,Jiang H,MacArthur MR,Gao X,Kong Y,Chouchani ET,Locasale JW,Snyder NW,Horng T

Sun Sep 01 00:00:00 UTC 2019

Obesity and steatosis promotes mitochondrial remodeling that enhances respiratory capacity in the liver of ob/ob mice.

FEBS letters

Garcia J,Decker CW,Sanchez SJ,Ouk JM,Siu KM,Han D

Thu Mar 01 00:00:00 UTC 2018

Obesity and steatosis promotes mitochondrial remodeling that enhances respiratory capacity in the liver of ob/ob mice.

FEBS letters

Garcia J,Decker CW,Sanchez SJ,Ouk JM,Siu KM,Han D

Thu Mar 01 00:00:00 UTC 2018

Mitochondrial remodeling in the liver following chronic alcohol feeding to rats.

Free radical biology & medicine

Han D,Johnson HS,Rao MP,Martin G,Sancheti H,Silkwood KH,Decker CW,Nguyen KT,Casian JG,Cadenas E,Kaplowitz N

Sun Jan 01 00:00:00 UTC 2017

Mitochondrial remodeling in the liver following chronic alcohol feeding to rats.

Free radical biology & medicine

Han D,Johnson HS,Rao MP,Martin G,Sancheti H,Silkwood KH,Decker CW,Nguyen KT,Casian JG,Cadenas E,Kaplowitz N

Sun Jan 01 00:00:00 UTC 2017

A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia.

European journal of human genetics : EJHG

Joshi M,Eagan J,Desai NK,Newton SA,Towne MC,Marinakis NS,Esteves KM,De Ferranti S,Bennett MJ,McIntyre A,Beggs AH,Berry GT,Agrawal PB

Wed Oct 01 00:00:00 UTC 2014

A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia.

European journal of human genetics : EJHG

Joshi M,Eagan J,Desai NK,Newton SA,Towne MC,Marinakis NS,Esteves KM,De Ferranti S,Bennett MJ,McIntyre A,Beggs AH,Berry GT,Agrawal PB

Wed Oct 01 00:00:00 UTC 2014

A refined analysis of superoxide production by mitochondrial sn-glycerol 3-phosphate dehydrogenase.

The Journal of biological chemistry

Orr AL,Quinlan CL,Perevoshchikova IV,Brand MD

Fri Dec 14 00:00:00 UTC 2012

A refined analysis of superoxide production by mitochondrial sn-glycerol 3-phosphate dehydrogenase.

The Journal of biological chemistry

Orr AL,Quinlan CL,Perevoshchikova IV,Brand MD

Fri Dec 14 00:00:00 UTC 2012

生物信息学

蛋白别名: FAD-linked glycerol-3-phosphate dehydrogenase; glycerol phosphate dehydrogenase 1, mitochondrial; Glycerol-3-phosophate dehydrogenase 2 (mitochondrial); glycerol-3-phosphate dehydrogenase 2 (mitochondrial); glycerol-3-phosphate dehydrogenase 2, mitochondrial; Glycerol-3-phosphate dehydrogenase, mitochondrial; GPD-M; GPDH-M; mGDH; mitochondrial glycerol-3-phosphate dehydrogenase; mitochondrial glycerophosphate dehydrogenase; mitohondrial glycerophosphate dehydrogenase gene; mtGPD; mtGPDH gene, promoter region and alternative transcripts; Protein TISP38; testicular tissue protein Li 76

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基因别名: AA408484; AI448216; AU021455; AW494132; GDH2; Gdm1; Gpd-m; GPD2; GPDH; Gpdh-m; GPDM; mGPDH; mtGPDH; TISP38

UniProt ID: (Human) P43304, (Mouse) Q64521, (Rat) P35571

Entrez Gene ID: (Human) 2820, (Mouse) 14571, (Rat) 25062

功能

glycerol-3-phosphate dehydrogenase activity calcium ion binding sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity glycerol-3-phosphate dehydrogenase [NAD+] activity oxidoreductase activity metal ion binding sn-glycerol-3-phosphate:ubiquinone oxidoreductase activity dehydrogenase

参与通路

glycerol-3-phosphate metabolic process gluconeogenesis glycerol catabolic process multicellular organism growth camera-type eye development cellular lipid metabolic process oxidation-reduction process NADH metabolic process