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GPR109 Antibody (12-4779-42) in Flow
Normal human peripheral blood were stained with Anti-Human CD16 PerCP-eFluor® 710 (Product # 46-0168-42), Anti-Human CCR3 APC (Product # 17-1939-42), and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human GPR109 PE (purple histogram). CD16-CCR3+ or CD16+CCR3- (Neutrophils) cells in the granulocyte gate were used for analysis.
产品信息
12-4779-42
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
4NZBRGO
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2688255
产品详细信息
This 4NZBRGO antibody has not been tested for reactivity with human GPR109B.
Applications Reported: This 4NZBRGO antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 4NZBRGO antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
GPR109A (also known as HCA2, HM74a, and NIACR1) is a 42kDa G-protein coupled receptor that belongs to a family of three hydroxyl-carboxylic acid receptors, which also include GPR109B and GPR81. GPR109A has two known ligands: niacin and butyrate (or its derivative 3-hydroxybutyrate). The endogenous ligands for GPR109B and GPR81 are 3-hydroxyl-octanoic acid and 2-hydroxy-propanoate (lactate), respectively. GPR109A is expressed on adipocytes, neutrophils, Langerhans cells, keratinocytes, intestinal epithelial cells, and intestinal colonic macrophages. Niacin, also known as nicotinic acid or vitamin B3, is known to improve cholesterol metabolism (LDL/HDL ratio) and reduce inflammation. It has been demonstrated that this effect is mediated through GPR109A. Notably, GPR109A also has an essential role in suppressing inflammation and cancer in the colon. Both GPR109A ligands are produced by colonic commensal bacteria. In mice, GPR109A expression on intestinal epithelial cells and DCs/macrophages contributes to the suppression of colitis and colon cancer. In humans, GPR109A is silenced in colon carcinoma cells by DNA methylation, which increases inflammation in the intestinal tract.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: G protein-coupled receptor 109A; G protein-coupled receptor HM74a; G-protein coupled receptor 109A; G-protein coupled receptor HM74A; hydroxy-carboxylic acid receptor 2; Hydroxycarboxylic acid receptor 2; Niacin receptor 1; Nicotinic acid receptor; PUMA-G. GPR109
基因别名: GPR109A; HCA2; HCAR2; HM74A; HM74b; NIACR1; Puma-g; PUMAG
UniProt ID: (Human) Q8TDS4
Entrez Gene ID: (Human) 338442
