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Application
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GPR177 Antibody (PA5-100549) in IHC (P)
Immunohistochemistry analysis of GPR177 in rat kidney tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GPR177 polyclonal antibody (Product # PA5-100549) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-100549
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human WLS(Accession Q5T9L3), corresponding to amino acid residues F473-T523.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2850058
Product Specific Information
Antibody detects endogenous levels of total GPR177.
Target Information
Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: evenness interrupted homolog; EVI; G protein-coupled receptor 177; Integral membrane protein GPR177; Protein evenness interrupted homolog; Protein wntless homolog; Putative NF-kappa-B-activating protein 373; putative NFkB activating protein 373
Gene Aliases: 5031439A09Rik; AI173978; AI987742; C1orf139; EVI; GPR177; mig-14; MRP; UNQ85/PRO18667; WLS
UniProt ID: (Human) Q5T9L3, (Mouse) Q6DID7, (Rat) Q6P689
Entrez Gene ID: (Human) 79971, (Mouse) 68151, (Rat) 362065
mesoderm formation
multicellular organism development
anterior/posterior axis specification
Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
biological_process
intracellular protein transport
positive regulation of Wnt signaling pathway
midbrain development
hindbrain development
exocrine pancreas development
positive regulation of I-kappaB kinase/NF-kappaB signaling
Wnt protein secretion
positive regulation of Wnt protein secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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