Invitrogen

GPX1 Recombinant Rabbit Monoclonal Antibody (5H1L9)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 篇参考文献

查看其他20个GPX1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

图: 1 / 3

GPX1 Antibody (702762) in WB

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of L6 (Lane 1), HT-29 (Lane 2) and tissue extract of Rat Liver (Lane 3) and Mouse Liver (Lane 4). The blots were probed with Anti-GPX1 Recombinant Rabbit Monoclonal Antibody (Product # 702762,1:200 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 22 kDa band corresponding to GPX1 was observed across the cell lines and tissue tested.

Product Image Gallery

GPX1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with GPX1 siRNA and decrease in signal intensity was observed in Western blot application using Anti-GPX1 Recombinant Rabbit Monoclonal Antibody (Product # 702762). {KD}

产品信息

702762

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

5H1L9

抗原

Peptides corresponding to human GPX1 [1) aa15-aa34, 2) aa129-aa144]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2723217

产品详细信息

This antibody is predicted to react with Monkey, Pig, Bovine.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

N-Acetylcysteine Enhances the Recovery of Ischemic Limb in Type-2 Diabetic Mice.

Antioxidants (Basel, Switzerland)

Zhu Q,Liu X,Zhu Q,Liu Z,Yang C,Wu H,Zhang L,Xia X,Wang M,Hao H,Cui Y,Zhang G,Hill MA,Flaker GC,Zhou S,Liu Z

702762 was used in Western Blotting to indicate that NAC treatment enhanced the recovery of blood flow and mechanical function in ischemic limbs in T2D mice in association with improved tissue redox/inflammatory status and insulin resistance.

Tue May 31 00:00:00 EDT 2022

Combination of Antioxidant Enzyme Overexpression and N-Acetylcysteine Treatment Enhances the Survival of Bone Marrow Mesenchymal Stromal Cells in Ischemic Limb in Mice With Type 2 Diabetes.

Journal of the American Heart Association

Zhu Q,Hao H,Xu H,Fichman Y,Cui Y,Yang C,Wang M,Mittler R,Hill MA,Cowan PJ,Zhang G,He X,Zhou S,Liu Z

702762 was used in Western Blotting to conclude that simultaneous suppression of reactive oxygen species from transplanted bMSCs and host tissue could additively enhance bMSC survival in diabetic ischemic limb with increased therapeutic efficacy in diabetes.

Tue Oct 05 00:00:00 EDT 2021

生物信息学

蛋白别名: Cellular glutathione peroxidase; Glutathione peroxidase 1; GPx-1; HGNC:4553; MGC14399; MGC88245; OTTHUMP00000210766; Phospholipid-hydroperoxide glutathione peroxidase GPX1; Selenium-dependent glutathione peroxidase 1

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基因别名: AI195024; AL033363; CGPx; Gpx; GPx-1; GPX1; GPXD; GSHPx; GSHPx-1; GSHPX1

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UniProt ID: (Human) P07203, (Rat) Q91WZ5, (Mouse) P11352

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Entrez Gene ID: (Human) 2876, (Rat) 24404, (Mouse) 14775

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功能

glutathione peroxidase activity selenium binding SH3 domain binding glutathione binding phospholipid-hydroperoxide glutathione peroxidase activity peroxidase activity protein binding oxidoreductase activity peroxidase

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