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GPX4 Antibody (PA5-102521) in ICC/IF
Immunofluorescent analysis of GPX4 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and GPX4 polyclonal antibody (Product # PA5-102521) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-102521
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human GPX4(Accession P36969), corresponding to amino acid residues I170-F197.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2851923
产品详细信息
Antibody detects endogenous levels of total GPX4.
靶标信息
Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Glutathione peroxidase 4; GPx-4; HGNC:4556; PHGPx; phospholipid hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase GPX4; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxide glutathione peroxidase, nuclear; sperm nuclei glutathione peroxidase; sperm nucleus glutathione peroxidase
基因别名: GPx-4; GPX4; GSHPx-4; MCSP; mtPHGPx; PHGPx; SMDS; snGPx; snPHGPx
UniProt ID: (Human) P36969, (Mouse) O70325, (Rat) P36970
Entrez Gene ID: (Human) 2879, (Mouse) 625249, (Rat) 29328
phospholipid metabolic process
response to oxidative stress
multicellular organism development
lipoxygenase pathway
oxidation-reduction process
cellular oxidant detoxification
chromatin organization
glutathione metabolic process
spermatogenesis
hydrogen peroxide catabolic process
regulation of inflammatory response
aging
response to estradiol
