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GRK3 Antibody (PA5-112350) in ICC/IF
Immunocytochemichal analysis of GRK3 in HepG2 cells using a Polyclonal antibody (Product # PA5-112350) at a dilution of 1:66. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
产品信息
PA5-112350
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human Beta-adrenergic receptor kinase 2 protein (343-480AA)
偶联物
形式
Liquid
浓度
3.93 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% ProClin 300
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
RRID
AB_2867086
靶标信息
ADRBK2, or adrenergic beta receptor kinase 2 is a serine/threonine kinase that is thought to act as a regulator of receptor function. Overall, the ADRBK2 enzyme, also known as GRK3, has 85% amino acid similarity with ADRBK1, with the protein kinase catalytic domain having 95% similarity. The ADRBK2 mRNA is approximately 8 kilobases with a distribution similar to that of ADRBK1. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: adrenergic, beta, receptor kinase 2; Beta-adrenergic receptor kinase 2; Beta-ARK-2; G protein-coupled receptor kinase 3; G-protein-coupled receptor kinase 3
基因别名: ADRBK2; BARK2; GRK3
UniProt ID: (Human) P35626
Entrez Gene ID: (Human) 157
