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GRP94 Antibody (PA5-27860) in ICC/IF
GRP94 Polyclonal Antibody detects GRP94 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GRP94 protein stained by GRP94 Polyclonal Antibody (Product # PA5-27860) diluted at 1:500. Blue: Hoechst 33342 staining.
产品信息
PA5-27860
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 1 and 366 of Human GRP94
偶联物
形式
Liquid
浓度
0.95 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2545336
产品详细信息
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3, endothelial cells.
Predicted reactivity: Mouse (98%), Rat (97%), Zebrafish (84%), Xenopus laevis (84%), Dog (99%), Pig (99%), Chicken (92%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
GRP94 is a 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. Expression of GRP94 suppressed A23187-induced apoptosis and stabilized calcium homeostasis. GRP94 is expressed in melanoma or liver metastases of colon carcinoma cells, human gastric carcinoma BGC-823 cells.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 94 kDa glucose-regulated protein; Endoplasmic reticulum resident protein 99; Endoplasmin; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; ERp99; gp96 homolog; GRP-94; Heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; Heat shock protein family C member 4; Polymorphic tumor rejection antigen 1; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96
基因别名: ECGP; endoplasmin; ERp99; GP96; GRP94; HEL-S-125m; HEL35; HSP90B1; HSPC4; TA-3; Targ2; Tra-1; TRA1
UniProt ID: (Human) P14625, (Mouse) P08113
Entrez Gene ID: (Human) 7184, (Mouse) 22027
response to hypoxia
toll-like receptor signaling pathway
receptor-mediated endocytosis
protein transport
ER-associated ubiquitin-dependent protein catabolic process
retrograde protein transport, ER to cytosol
actin rod assembly
protein folding in endoplasmic reticulum
response to endoplasmic reticulum stress
ATF6-mediated unfolded protein response
negative regulation of apoptotic process
regulation of phosphoprotein phosphatase activity
sequestering of calcium ion
cellular response to ATP
protein folding
response to stress
