Invitrogen

GSDMD Polyclonal Antibody

GSDMD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GSDMD Antibody (PA5-119680) in IHC (P)

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human prostate carcinoma tissue using GSDMD Polyclonal Antibody (Product # PA5-119680). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the GSDMD antibody at a... View More

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产品信息

PA5-119680

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:1,000

流式细胞分析 (Flow)

1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein within human Gasdermin D aa 100-300

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2913253

产品详细信息

Positive Control: SiHa cell lysates, human kidney tissue lysates, SiHa, rat stomach tissue, human tonsil tissue, human prostate carcinoma tissue, human esophagus tissue, Mouse colon tissue.

Subcellular Location: Cytosol. Secreted. Plasma membrane.

靶标信息

GSDMD (gasdermin-D) promotes pyroptosis in response to microbial infection and danger signals. It is produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as, non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, GSDMD moves to the plasma membrane where it strongly binds to inner leaflet lipids including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-biphosphate, phosphatidylinositol (3,4,5)-biphosphate, phosphatidic acid, and phosphatidylserine. GSDMD also exhibits bactericidal activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。