Proteintech

GSDMD Monoclonal Antibody (2E6C7)

10 篇参考文献

查看其他24个GSDMD抗体

抗体检测数据 (10)

GSDMD Antibody in Immunocytochemistry (ICC/IF)

GSDMD Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 10

GSDMD Antibody (66387-1-IG) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 66387-1-IG (GSDMD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

GSDMD Antibody in Immunocytochemistry (ICC/IF)

GSDMD Antibody in Western Blot (WB)

GSDMD Antibody in Western Blot (WB)

GSDMD Antibody in Western Blot (WB)

GSDMD Antibody in Western Blot (WB)

GSDMD Antibody in Western Blot (WB)

GSDMD Antibody in Western Blot (WB)

GSDMD Antibody in Western Blot (WB)

GSDMD Antibody in Western Blot (WB)

GSDMD Antibody in Western Blot (WB)

Product Image Gallery

GSDMD Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 66387-1-IG (GSDMD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

GSDMD Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 66387-1-Ig) GSDMD antibody at dilution of 1:20,000 incubated at room temperature for 1.5 hours.

GSDMD Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 66387-1-Ig) GSDMD antibody at dilution of 1:10,000 incubated at room temperature for 1.5 hours.

GSDMD Antibody in Western Blot (WB) — Human small intestine tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

GSDMD Antibody in Western Blot (WB) — Human colon tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

GSDMD Antibody in Western Blot (WB) — COLO 320 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

GSDMD Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 66387-1-IG ( GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

GSDMD Antibody in Western Blot (WB) — EC109 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

GSDMD Antibody in Western Blot (WB) — Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

GSDMD Antibody in Western Blot (WB) — A431 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

产品信息

66387-1-IG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:6,000

查看9篇已发表文章 9篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:500

-

不适用 (N/A)

-

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

2E6C7

抗原

GSDMD Fusion Protein Ag16827 (135-484 aa encoded by BC008904)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

Immunogen sequence: HERHLRQPE HKVLQQLRSR GDNVYVVTEV LQTQKEVEVT RTHKREGSGR FSLPGATCLQ GEGQGHLSQK KTVTIPSGST LAFRVAQLVI DSDLDVLLFP DKKQRTFQPP ATGHKRSTSE GAWPQLPSGL SMMRCLHNFL TDGVPAEGAF TEDFQGLRAE VETISKELEL LDRELCQLLL EGLEGVLRDQ LALRALEEAL EQGQSLGPVE PLDGPAGAVL ECLVLSSGML VPELAIPVVY LLGALTMLSE TQHKLLAEAL ESQTLLGPLE LVGSLLEQSA PWQERSTMSL PPGLLGNSWG EGAPAWVLLD ECGLELGEDT PHVCWEPQAQ GRMCALYASL ALLSGLSQEP H (135-484 aa encoded by BC008904)

靶标信息

GSDMD (gasdermin-D) promotes pyroptosis in response to microbial infection and danger signals. It is produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as, non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, GSDMD moves to the plasma membrane where it strongly binds to inner leaflet lipids including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-biphosphate, phosphatidylinositol (3,4,5)-biphosphate, phosphatidic acid, and phosphatidylserine. GSDMD also exhibits bactericidal activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

MALAT1 knockdown alleviates the pyroptosis of microglias in diabetic cerebral ischemia via regulating STAT1 mediated NLRP3 transcription.

Molecular medicine (Cambridge, Mass.)

Zhao N,Hua W,Liu Q,Wang Y,Liu Z,Jin S,Wang B,Pang Y,Qi J,Song Y

Mon Apr 03 00:00:00 EDT 2023

CBL0137 activates ROS/BAX signaling to promote caspase-3/GSDME-dependent pyroptosis in ovarian cancer cells.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Yang C,Wang ZQ,Zhang ZC,Lou G,Jin WL

Mon May 01 00:00:00 EDT 2023

Bone marrow-derived mesenchymal stem cell-conditioned medium ameliorates diabetic foot ulcers in rats.

Clinics (Sao Paulo, Brazil)

Xu YF,Wu YX,Wang HM,Gao CH,Xu YY,Yan Y

Thu Mar 30 00:00:00 EDT 2023

Involvement of pyroptosis pathway in epicardial adipose tissue - myocardium axis in experimental heart failure with preserved ejection fraction.

Biochemical and biophysical research communications

Xia YY,Shi Y,Li Z,Li H,Wu LD,Zhou WY,Gu Y,Ling ZY,Zhang JX,Chen SL

Sun Dec 25 00:00:00 EST 2022

Isoxanthohumol, a component of Sophora flavescens, promotes the activation of the NLRP3 inflammasome and induces idiosyncratic hepatotoxicity.

Journal of ethnopharmacology

Lin L,Chen Y,Li Q,Xu G,Ding K,Ren L,Shi W,Wang Y,Li Z,Dai W,Wei Z,Yang Y,Bai Z,Xiao X

Tue Mar 01 00:00:00 EST 2022

HIV-induced bystander cell death in astrocytes requires cell-to-cell viral transmission.

Journal of neurochemistry

Cevallos C,Ojeda DS,Sánchez L,Urquiza J,Delpino MV,Quarleri J

Tue Nov 01 00:00:00 EDT 2022

Pyroptosis correlates with tumor immunity and prognosis.

Communications biology

Lou X,Li K,Qian B,Li Y,Zhang D,Cui W

Tue Sep 06 00:00:00 EDT 2022

Acquiring high expression of suilysin enable non-epidemic Streptococccus suis to cause streptococcal toxic shock-like syndrome (STSLS) through NLRP3 inflammasome hyperactivation.

Emerging microbes & infections

Xu L,Lin L,Lu X,Xiao P,Liu R,Wu M,Jin M,Zhang A

Wed Dec 01 00:00:00 EST 2021

An NLRP3 inflammasome-triggered cytokine storm contributes to Streptococcal toxic shock-like syndrome (STSLS).

PLoS pathogens

Lin L,Xu L,Lv W,Han L,Xiang Y,Fu L,Jin M,Zhou R,Chen H,Zhang A

Sat Jun 01 00:00:00 EDT 2019

Cell Death Is Counteracted by Mitophagy in HIV-Productively Infected Astrocytes but Is Promoted by Inflammasome Activation Among Non-productively Infected Cells.

Frontiers in immunology

Ojeda DS,Grasso D,Urquiza J,Till A,Vaccaro MI,Quarleri J

Wed Oct 02 00:00:00 EDT 2019

生物信息学

蛋白别名: gasdermin domain containing 1; Gasdermin domain-containing protein 1; Gasdermin-D

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基因别名: DF5L; DFNA5L; FKSG10; GSDMD; GSDMDC1

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UniProt ID: (Human) P57764

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Entrez Gene ID: (Human) 79792

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protein binding

参与通路

inflammatory response cellular response to extracellular stimulus innate immune response positive regulation of interleukin-1 beta secretion pyroptosis