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GST Antibody (740007MB) in WB
Western blot was performed using GST tag Recombinant Mouse monoclonal antibody (GST 3-4C), Biotin (Product # 740007MB) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: Untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), H3-GST, 40 µg (Lane 3), H3-GST, 30 µg (Lane 4), H3-GST, 20 µg (Lane 5), GST-p65, 40 µg (Lane 6), GST-p65, 30 µg (Lane 7), GST-p65, 20 µg (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then tran... View More
产品信息
740007MB
产品规格
种属反应
Tag
宿主/亚型
Mouse
/ IgG2b, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
GST 3-4C
抗原
GST protein from Schistosoma japonicum expressed from the pGEX vector.
偶联物
Biotin
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein A/G
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_3074179
产品详细信息
GST Tag recombinant mouse monoclonal antibody conjugated with Biotin specifically recognizes GST Tag at both C and N-terminus of proteins.
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Glutathione-S-Transferase; GST protein; GST Tag
