Invitrogen

GTF2I Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他22个GTF2I抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (4)
高级验证 (1)

GTF2I Antibody in Immunocytochemistry (ICC/IF)

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GTF2I Antibody (PA5-17642) in ICC/IF

Immunofluorescence analysis of GTF2I was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GTF2I Polyclonal Antibody (Product # PA5-17642) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More

GTF2I Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GTF2I Antibody — Antibody specificity was demonstrated by siRNA mediated KD of target protein. HeLa cells were transfected with GTF2I siRNA and reduction of signal was observed in Western Blot using GTF2I Polyclonal Antibody (Product # PA5-17642). {KD}

产品信息

PA5-17642

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:400

免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

1:50

产品规格

种属反应

Human, Mouse, Non-human primate

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues of human TFII-I

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

15 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10981419

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

This gene encodes a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11. 23. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 7, 13 and 21.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: BAP-135; Bruton tyrosine kinase-associated protein 135; BTK-associated protein 135; BTK-associated protein, 135kD; General transcription factor II-I; GTFII-I; OTTHUMP00000209509; SPIN; SRF-Phox1-interacting protein; tfiii; Williams-Beuren syndrome chromosomal region 6 protein; Williams-Beuren syndrome chromosome region 6

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基因别名: 6030441I21Rik; BAP-135; BAP135; BTKAP1; DIWS; Diws1t; GTF2I; GTFII-I; IB291; SPIN; TFII-I; WBS; WBSCR6

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UniProt ID: (Human) P78347, (Mouse) Q9ESZ8

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Entrez Gene ID: (Human) 2969, (Mouse) 14886

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功能

RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding mitogen-activated protein kinase binding general transcription factor

参与通路

regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter signal transduction transition between slow and fast fiber negative regulation of angiogenesis negative regulation of cytosolic calcium ion concentration transcription, DNA-templated regulation of transcription, DNA-templated embryo development