Invitrogen

GYS1 Monoclonal Antibody (J.18.5)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 篇参考文献

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (4)
高级验证 (1)

GYS1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

GYS1 Antibody (MA5-15022) in ICC/IF

Immunofluorescence analysis of GYS1 was performed using 70% confluent log phase HSkMs cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GYS1 Monoclonal Antibody (Product # MA5-15022) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a diluti... View More

GYS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

GYS1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with GYS1 siRNA and loss of signal was observed in Western Blot using Anti- GYS1 Monoclonal Antibody (Product # MA5-15022). {KD}

产品信息

MA5-15022

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

1:25

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

J.18.5

抗原

Synthetic peptide corresponding to the sequence of human glycogen synthase

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

24 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_10986224

产品详细信息

GYS1 Monoclonal Antibody (J.18.5) detects endogenous levels of total muscle and liver glycogen synthase protein.

It is not recommended to aliquot this antibody.

靶标信息

Glycogen is a polysaccharide of glucose and serves as an energy storage in mammalian muscle and liver. Glycogen synthase catalyzes the rate-limiting step of glycogen biosynthesis through the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Glycogen synthase has two major isoforms in mammals: muscle isoform (GYS1) and liver isoform (GYS2).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Targeting SLC7A11 improves efferocytosis by dendritic cells and wound healing in diabetes.

Nature

Maschalidi S,Mehrotra P,Keçeli BN,De Cleene HKL,Lecomte K,Van der Cruyssen R,Janssen P,Pinney J,van Loo G,Elewaut D,Massie A,Hoste E,Ravichandran KS

MA5-15022 was used in Western Blotting to reveal that the membrane transporter SLC7A11 acts as a molecular brake on efferocytosis, the process by which dying cells are removed, and that inhibiting SLC7A11 function can accelerate wound healing.

Wed Jun 01 00:00:00 EDT 2022

生物信息学

蛋白别名: Glycogen [starch] synthase, liver; Glycogen [starch] synthase, muscle; Glycogen synthase 1; glycogen synthase 1 (muscle); Glycogen synthase 2; glycogen synthase 2 (liver); glycogen synthase 3, brain; glycogen synthase, liver

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基因别名: BC021322; GLYSN; GSY; GYS; GYS1; GYS2; Gys3; LGS; MGS