Invitrogen

Galectin 1 Polyclonal Antibody, PeproTech®

Galectin 1 Antibody in Western Blot (WB)

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Galectin 1 Antibody (500-P210-1MG) in WB

Western Blot: To detect Human Galectin-1 by Western Blot analysis Galectin 1 Polyclonal Antibody (Product # 500-P210-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Galectin-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Galectin 1 Antibody in Immunohistochemistry (IHC)

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产品信息

500-P210-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

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体外检测 (IV)

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其他PubMed (Misc)

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免疫染色 (IS)

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产品规格

种属反应

Human

已发表种属

Chicken, Hamster, Human, Mouse

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human Galectin-1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2929826

产品详细信息

AA Sequence of recombinant protein: ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Galectin-1. Anti-Human Galectin-1-specific antibody was purified by affinity chromatography employing an immobilized Human Galectin-1 matrix.

Sandwich ELISA: To detect Human Galectin-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Galectin-1 (500-P210Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Galectin-1.

Western Blot: To detect Human Galectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Galectin-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

Galectin-1 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-1can be either monomeric or homodimeric and is found in a wide variety of cells and tissue types. Galectin-1 can control cell growth, proliferation, induce apoptosis of activated T cells while it can also modulate cytokine secretion or inhibit pro-inflammatory cytokine production. Galectin-1 plays an important role in acute and chronic inflammation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Stromal Galectin-1 Promotes Colorectal Cancer Cancer-Initiating Cell Features and Disease Dissemination Through SOX9 and β-Catenin: Development of Niche-Based Biomarkers.

Frontiers in oncology

Peng KY,Jiang SS,Lee YW,Tsai FY,Chang CC,Chen LT,Yen BL

500-P210 was used in Enzyme-linked immunosorbent assay to elucidate the molecular interactions between stromal and CRC cells for the development of prognostically relevant biomarkers.

Wed Sep 29 00:00:00 EDT 2021

Chemokines modulate glycan binding and the immunoregulatory activity of galectins.

Communications biology

Sanjurjo L,Schulkens IA,Touarin P,Heusschen R,Aanhane E,Castricum KCM,De Gruijl TD,Nilsson UJ,Leffler H,Griffioen AW,Elantak L,Koenen RR,Thijssen VLJL

Mon Dec 20 00:00:00 EST 2021

Indoleamine 2,3-Dioxygenase Expression Pattern in the Tumor Microenvironment Predicts Clinical Outcome in Early Stage Cervical Cancer.

Frontiers in immunology

Heeren AM,van Dijk I,Berry DRAI,Khelil M,Ferns D,Kole J,Musters RJP,Thijssen VL,Mom CH,Kenter GG,Bleeker MCG,de Gruijl TD,Jordanova ES

500-P210 was used in Immunohistochemistry-immunofluorescence to indicate that the serum kyn/trp ratio and IDO expression in primary tumor samples are not clear-cut biomarkers for prognosis and stratification of patients with early stage cervical cancer for clinical trials implementing IDO inhibitors.

Wed Nov 20 00:00:00 EST 2019

Opposite feedback from mTORC1 to H-ras and K-ras4B downstream of SREBP1.

Scientific reports

Posada IMD,Lectez B,Siddiqui FA,Oetken-Lindholm C,Sharma M,Abankwa D

Mon Aug 21 00:00:00 EDT 2017

Galectin-1 dimers can scaffold Raf-effectors to increase H-ras nanoclustering.

Scientific reports

Blaževitš O,Mideksa YG,Šolman M,Ligabue A,Ariotti N,Nakhaeizadeh H,Fansa EK,Papageorgiou AC,Wittinghofer A,Ahmadian MR,Abankwa D

Mon Apr 18 00:00:00 EDT 2016

Galectin-1 dimers can scaffold Raf-effectors to increase H-ras nanoclustering.

Scientific reports

Blaževitš O,Mideksa YG,Šolman M,Ligabue A,Ariotti N,Nakhaeizadeh H,Fansa EK,Papageorgiou AC,Wittinghofer A,Ahmadian MR,Abankwa D

Mon Apr 18 00:00:00 EDT 2016

ASPP2 Is a Novel Pan-Ras Nanocluster Scaffold.

PloS one

Posada IM,Serulla M,Zhou Y,Oetken-Lindholm C,Abankwa D,Lectez B

500-P210 was used in Immunocytochemistry to investigate the effects of ASPP2 Ras nanoclustering.

Fri Jul 21 00:00:00 EDT 2017

ASPP2 Is a Novel Pan-Ras Nanocluster Scaffold.

PloS one

Posada IM,Serulla M,Zhou Y,Oetken-Lindholm C,Abankwa D,Lectez B

500-P210 was used in Immunocytochemistry to investigate the effects of ASPP2 Ras nanoclustering.

Fri Jul 21 00:00:00 EDT 2017

Glioma-derived galectin-1 regulates innate and adaptive antitumor immunity.

International journal of cancer

Verschuere T,Toelen J,Maes W,Poirier F,Boon L,Tousseyn T,Mathivet T,Gerhardt H,Mathieu V,Kiss R,Lefranc F,Van Gool SW,De Vleeschouwer S

500-P210 was used in Immunohistochemistry to use the orthotopic GL261 mouse high-grade glioma model to study whether the absence or presence of galectin-1 within the glioma microenvironment causes qualitative or quantitative differences in innate and/or adaptive antitumor immune responses.

Sat Feb 15 00:00:00 EST 2014

Glioma-derived galectin-1 regulates innate and adaptive antitumor immunity.

International journal of cancer

Verschuere T,Toelen J,Maes W,Poirier F,Boon L,Tousseyn T,Mathivet T,Gerhardt H,Mathieu V,Kiss R,Lefranc F,Van Gool SW,De Vleeschouwer S

Sat Feb 15 00:00:00 EST 2014

Galectin-1 prolongs survival of mouse liver allografts from Flt3L-pretreated donors.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Ye Y,Yan S,Jiang G,Zhou L,Xie H,Xie X,Yu X,Ding Y,Tian J,Dai Y,Zheng S

Fri Mar 01 00:00:00 EST 2013

Vascular regrowth following photodynamic therapy in the chicken embryo chorioallantoic membrane.

Angiogenesis

Nowak-Sliwinska P,van Beijnum JR,van Berkel M,van den Bergh H,Griffioen AW

Wed Dec 01 00:00:00 EST 2010

AP1-dependent galectin-1 expression delineates classical hodgkin and anaplastic large cell lymphomas from other lymphoid malignancies with shared molecular features.

Clinical cancer research : an official journal of the American Association for Cancer Research

Rodig SJ,Ouyang J,Juszczynski P,Currie T,Law K,Neuberg DS,Rabinovich GA,Shipp MA,Kutok JL

Sun Jun 01 00:00:00 EDT 2008

T cell apoptosis at the maternal-fetal interface in early human pregnancy, involvement of galectin-1.

Proceedings of the National Academy of Sciences of the United States of America

Kopcow HD,Rosetti F,Leung Y,Allan DS,Kutok JL,Strominger JL

Tue Nov 25 00:00:00 EST 2008

Galectin-1 mediated suppression of Epstein-Barr virus specific T-cell immunity in classic Hodgkin lymphoma.

Blood

Gandhi MK,Moll G,Smith C,Dua U,Lambley E,Ramuz O,Gill D,Marlton P,Seymour JF,Khanna R

Wed Aug 15 00:00:00 EDT 2007

生物信息学

蛋白别名: 14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14kDa; DKFZp686E23103; Gal-1; Galaptin; Galectin-1; Galectin1; H-Galectin-1; HBL; HLBP14; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside binding soluble 1; lectin, galactoside-binding, soluble, 1; pm12; Putative MAPK-activating protein PM12; S-Lac lectin 1

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基因别名: GAL1; GBP; LGALS1

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UniProt ID: (Human) P09382

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Entrez Gene ID: (Human) 3956

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功能

glycoprotein binding signal transducer activity protein binding lactose binding protein homodimerization activity laminin binding poly(A) RNA binding extracellular matrix protein

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