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AA Sequence of recombinant protein: ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Galectin-1. Anti-Human Galectin-1-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hGalectin-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Galectin-1 (500-P210) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hGalectin-1.
Western Blot: To detect hGalectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGalectin-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P210BT-1MG will be provided as 2 x 500 µg
Galectin-1 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-1can be either monomeric or homodimeric and is found in a wide variety of cells and tissue types. Galectin-1 can control cell growth, proliferation, induce apoptosis of activated T cells while it can also modulate cytokine secretion or inhibit pro-inflammatory cytokine production. Galectin-1 plays an important role in acute and chronic inflammation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。