Invitrogen

Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), PE, eBioscience™

Galectin 3 Antibody in Flow Cytometry (Flow)

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Galectin 3 Antibody (12-5301-82) in Flow

Staining of C57BL/6 bone marrow cells with Anti-Mouse CD11b FITC (Product # 11-0112-41) and Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.03 µg of Anti-Human/Mouse Galectin-3 PE (right). Total viable cells were used for analysis.

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产品信息

12-5301-82

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.06 µg/test

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产品规格

种属反应

Human, Mouse

已发表种属

Hamster, Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioM3/38 (M3/38)

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_842792

产品详细信息

Description: The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.

This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3).

Applications Reported: This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioM3/38 (M3/38) antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal macrophages. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Development of a Syrian hamster anti-PD-L1 monoclonal antibody enables oncolytic adenoviral immunotherapy modelling in an immunocompetent virus replication permissive setting.

Frontiers in immunology

Clubb JHA,Kudling TV,Girych M,Haybout L,Pakola S,Hamdan F,Cervera-Carrascon V,Hemmes A,Grönberg-Vähä-Koskela S,Santos JM,Quixabeira DCA,Basnet S,Heiniö C,Arias V,Jirovec E,Kaptan S,Havunen R,Sorsa S,Erikat A,Schwartz J,Anttila M,Aro K,Viitala T,Vattulainen I,Cerullo V,Kanerva A,Hemminki A

12-5301-82 was used in Flow cytometry/Cell sorting to develop an anti-Syrian hamster PD-L1 mAb to enable the evaluation of safety and efficacy, when combining anti-PD-L1 with an oncolytic adenovirus encoding tumour necrosis factor alpha (TNFα) and interleukin-2 (IL-2) (Ad5/3-E2F-D24-hTNFα-IRES-hIL-2 or TILT-123).

Mon Feb 27 00:00:00 EST 2023

Endocytic membrane repair by ESCRT-III controls antigen export to the cytosol during antigen cross-presentation.

Cell reports

Gros M,Segura E,Rookhuizen DC,Baudon B,Heurtebise-Chrétien S,Burgdorf N,Maurin M,Kapp EA,Simpson RJ,Kozik P,Villadangos JA,Bertrand MJM,Burbage M,Amigorena S

12-5301-82 was used in Immunohistochemistry-immunofluorescence to demonstrate that membrane repair proves crucial in containing antigen export to the cytosol and cross-presentation in cDCs.

Tue Aug 16 00:00:00 EDT 2022

Cell surface galectin-3 defines a subset of chemoresistant gastrointestinal tumor-initiating cancer cells with heightened stem cell characteristics.

Cell death & disease

Ilmer M,Mazurek N,Byrd JC,Ramirez K,Hafley M,Alt E,Vykoukal J,Bresalier RS

12-5301 was used in Flow cytometry/Cell sorting to examine the ability of Gal3 to characterise the cancer stem cell (CSC) phenotype, and identify a clinically important gastrointestinal cancer CSC population.

Thu Aug 11 00:00:00 EDT 2016

Maternal immune activation evoked by polyinosinic:polycytidylic acid does not evoke microglial cell activation in the embryo.

Frontiers in cellular neuroscience

Smolders S,Smolders SM,Swinnen N,Gärtner A,Rigo JM,Legendre P,Brône B

12-5301 was used in Flow cytometry/Cell sorting to indicate that activation of the fetal microglial cells is not likely to be responsible for the inflammation induced deficits in this model for neurodevelopmental disorders.

Mon Aug 24 00:00:00 EDT 2015

Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies.

The Journal of experimental medicine

Madireddi S,Eun SY,Lee SW,Nemčovičová I,Mehta AK,Zajonc DM,Nishi N,Niki T,Hirashima M,Croft M

12-5301 was used in Flow cytometry/Cell sorting to delineate the mechanism of action of agonist anti-4-1BB in suppressing autoimmune and allergic inflammation.

Mon Jun 30 00:00:00 EDT 2014

生物信息学

蛋白别名: 35 kDa lectin; advanced glycation end-product receptor 3; Carbohydrate-binding protein 35; CBP 35; CBP-30; Gal-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; Galectin-3; Galectin3; IgE-binding protein; L-29; L-31; L-34 galactoside-binding lectin; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; MAC-2 antigen

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基因别名: CBP35; GAL3; GALBP; GALIG; GBP; L-34; L31; LGALS2; LGALS3; Mac-2; MAC2

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UniProt ID: (Human) Q96J47

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Entrez Gene ID: (Human) 3958, (Mouse) 16854

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功能