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GluD1 Antibody (PA5-28301) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of GluD1 was performed in U-87 MG cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GluD1 Polyclonal Antibody (Product # PA5-28301) diluted at 1:500. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody. Blue: Hoechst 33342 staining.
产品信息
PA5-28301
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 64 and 366 of Human Glutamate Dehydrogenase
偶联物
形式
Liquid
浓度
0.07 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2545777
产品详细信息
Recommended positive controls: 293T, A431, HeLa, HepG2, THP1, Raw264.7.
Predicted reactivity: Mouse (99%), Rat (98%), Xenopus laevis (91%), Pig (99%), Sheep (99%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Glutamate dehydrogenase, GluD1, is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: epididymis tissue sperm binding protein Li 18mP; GDH 1; glutamate dehydrogenase (NAD(P)+); Glutamate dehydrogenase 1, mitochondrial; Memory-related gene 2 protein; MRG-2
基因别名: Ac2-281; AI118167; GDH; Gdh-X; GDH1; GLUD; GLUD1; Gludeha; Gludl; MRG-2
UniProt ID: (Human) P00367, (Mouse) P26443, (Rat) P10860
Entrez Gene ID: (Human) 2746, (Mouse) 14661, (Rat) 24399
glutamate biosynthetic process
glutamate catabolic process
glutamine metabolic process
cellular amino acid biosynthetic process
substantia nigra development
positive regulation of insulin secretion
oxidation-reduction process
tricarboxylic acid metabolic process
cellular amino acid metabolic process
response to aluminum ion
long-term memory
