Invitrogen

Glucagon Polyclonal Antibody

Glucagon Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Glucagon Antibody (PA5-13442) in IHC (P)

Immunohistochemistry analysis of Glucagon in formalin-fixed and paraffin-embedded human hepatocarcinoma. Samples were incubated with Glucagon polyclonal antibody (Product # PA5-13442) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Glucagon Antibody in Flow Cytometry (Flow)

Glucagon Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-13442

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:10-1:50

流式细胞分析 (Flow)

1:10-1:50

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产品规格

种属反应

Human, Mouse

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human GCG

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Size-exclusion - Dialysis, Ammonium sulfate precipitation

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2107206

靶标信息

Glucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogensis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or alpha cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Pathological manifestations of the glucagon-type peptide residue almost exclusively with the exsistence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. Glucagon-specific antibodies would prove useful as a cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in qualification of the hormone.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

DYRK1A inhibitors leucettines and TGF-β inhibitor additively stimulate insulin production in beta cells, organoids, and isolated mouse islets.

PloS one

Pucelik B,Barzowska A,Czarna A

PA5-13442 was used in Flow cytometry/Cell sorting to show that leucettines, known to be DYRK1A kinase inhibitors, can improve glucose-stimulated insulin secretion (GSIS) in rodent β-cells and isolated islets, as well as in hiPSC-derived β-cells islets.

Fri May 19 00:00:00 EDT 2023

DYRK1A inhibitors leucettines and TGF-β inhibitor additively stimulate insulin production in beta cells, organoids, and isolated mouse islets.

PloS one

Pucelik B,Barzowska A,Czarna A

Fri May 19 00:00:00 EDT 2023

CD4⁺ T cells regulate glucose homeostasis independent of adipose tissue dysfunction in mice.

European journal of immunology

Brinker G,Froeba J,Arndt L,Braune J,Hobusch C,Lindhorst A,Bechmann I,Gericke M

PA5-13442 was used in Western Blot to examine a direct impact of CD4-positive T cells on adipose tissue macrophages(ATM) activation, ATM proliferation and glucose homeostasis using an in vivo depletion model.

Tue Jun 01 00:00:00 EDT 2021

Influence of Cannabinoid Receptor Deficiency on Parameters Involved in Blood Glucose Regulation in Mice.

International journal of molecular sciences

Zibolka J,Wolf A,Rieger L,Rothgänger C,Jörns A,Lutz B,Zimmer A,Dehghani F,Bazwinsky-Wutschke I

PA5-13442 was used in Western Blotting to further investigate the impact of cannabinoid receptors on the parameters involved in insulin secretion and blood glucose recognition.

Thu Apr 30 00:00:00 EDT 2020

Influence of Cannabinoid Receptor Deficiency on Parameters Involved in Blood Glucose Regulation in Mice.

International journal of molecular sciences

Zibolka J,Wolf A,Rieger L,Rothgänger C,Jörns A,Lutz B,Zimmer A,Dehghani F,Bazwinsky-Wutschke I

PA5-13442 was used in Western Blotting to further investigate the impact of cannabinoid receptors on the parameters involved in insulin secretion and blood glucose recognition.

Thu Apr 30 00:00:00 EDT 2020

The Protective Role of Prenatal Alpha Lipoic Acid Supplementation against Pancreatic Oxidative Damage in Offspring of Valproic Acid-Treated Rats: Histological and Molecular Study.

Biology

Ghoneim FM,Alrefai H,Elsamanoudy AZ,Abo El-Khair SM,Khalaf HA

PA5-13442 was used in Immunohistochemistry to investigate the effect of prenatal administration of VPA on the pancreas of rat offspring and assess the potential protective role of ALA co-administration during pregnancy.

Thu Aug 20 00:00:00 EDT 2020

The Protective Role of Prenatal Alpha Lipoic Acid Supplementation against Pancreatic Oxidative Damage in Offspring of Valproic Acid-Treated Rats: Histological and Molecular Study.

Biology

Ghoneim FM,Alrefai H,Elsamanoudy AZ,Abo El-Khair SM,Khalaf HA

Thu Aug 20 00:00:00 EDT 2020

生物信息学

蛋白别名: glicentin-related polypeptide; GLP1/2; glucagon-like peptide 1; glucagon-like peptide 2; IL12p35; IL12P40; OXY; preproglucagon; Pro-glucagon

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基因别名: GCG; GLP-1; GLP1; GLP2; Glu; GRPP; PPG

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UniProt ID: (Human) P01275, (Mouse) P55095

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Entrez Gene ID: (Human) 2641, (Mouse) 14526

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功能

receptor binding hormone activity protein binding glucagon receptor binding identical protein binding

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