Invitrogen

Glucagon Monoclonal Antibody (ICACLS), eBioscience™

Glucagon Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Glucagon Antibody (14-9743-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human pancreas using 0.1 µg/mL of Anti-Glucagon Purified, followed by Anti-Mouse IgG Biotin, Streptavidin HRP and DAB visualization.Nuclei are counterstained with hematoxylin.

Glucagon Antibody in Immunohistochemistry (IHC)

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产品信息

14-9743-82

应用

建议稀释比

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

0.1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

ICACLS

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2572972

产品详细信息

Description: This ICACLS monoclonal antibody reacts with human, mouse, and rat glucagon. Glucagon is a peptide hormone secreted by the pancreatic alpha cells found within the islets of Langerhans. Glucagon functions as an antagonist to insulin and homeostatic regulation of blood glucose levels are maintained by the critical balance between these two hormones. Glucagon acts in the liver to convert glycogen to glucose by promoting gluconeogenesis and glycogenolysis. Secretion of glucagon is stimulated by hypoglycemia, epinephrine, acetylcholine, and cholecystokinin, while secretion is inhibited by insulin and somatostatin. Abnormally high levels of glucagon are found in pancreatic tumors.

Applications Reported: This ICACLS antibody has been reported for use in immunohistochemical staining, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This ICACLS antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 0.1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Glucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogensis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or alpha cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Pathological manifestations of the glucagon-type peptide residue almost exclusively with the exsistence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. Glucagon-specific antibodies would prove useful as a cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in qualification of the hormone.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Irisin-A Pancreatic Islet Hormone.

Biomedicines

Norman D,Drott CJ,Carlsson PO,Espes D

Published figure using Glucagon monoclonal antibody (Product # 14-9743-82) in Immunohistochemistry

Tue Jan 25 00:00:00 EST 2022

IL18 signaling causes islet β cell development and insulin secretion via different receptors on acinar and β cells.

Developmental cell

Zhang X,Luo S,Wang M,Huang Q,Fang W,Li J,Liu T,Zhang Y,Deng Z,Liu CL,Guan S,Ayala JE,Flavell RA,Kulkarni RN,Libby P,Guo J,Liu Z,Shi GP

14-9743-82 was used in Immunohistochemistry to report that IL18 is expressed on islet α cells, NCC on β cells, and IL18r on acinar cells in human and mouse pancreases.

Mon Jun 20 00:00:00 EDT 2022

IL18 signaling causes islet β cell development and insulin secretion via different receptors on acinar and β cells.

Developmental cell

Zhang X,Luo S,Wang M,Huang Q,Fang W,Li J,Liu T,Zhang Y,Deng Z,Liu CL,Guan S,Ayala JE,Flavell RA,Kulkarni RN,Libby P,Guo J,Liu Z,Shi GP

14-9743-82 was used in Immunohistochemistry to report that IL18 is expressed on islet α cells, NCC on β cells, and IL18r on acinar cells in human and mouse pancreases.

Mon Jun 20 00:00:00 EDT 2022

Pregestational diet transition to normal-fat diet avoids the deterioration of pancreatic β-cell function in male offspring induced by maternal high-fat diet.

The Journal of nutritional biochemistry

Liu Z,Ding Z,Lynch EC,McCauley N,Zhou Y,Zhang KK,Xie L

14-9743-82 was used in Immunohistochemistry to evaluate whether the adverse effects of maternal overnutrition on offspring β-cell function are reversible or preventable through preconception maternal diet interventions.

Tue Dec 01 00:00:00 EST 2020

生物信息学

蛋白别名: glicentin-related polypeptide; GLP1/2; glucagon; glucagon-like peptide 1; glucagon-like peptide 2; glucagon-like peptide-1; IL12p35; IL12P40; OXY; preproglucagon; Pro-glucagon

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基因别名: GCG; GLP-1; GLP1; GLP2; Glu; GRPP; PPG

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UniProt ID: (Human) P01275, (Mouse) P55095

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Entrez Gene ID: (Human) 2641, (Mouse) 14526, (Rat) 24952

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功能

receptor binding hormone activity protein binding glucagon receptor binding identical protein binding

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