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Application
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Glutaminase C (GAC) Antibody (PA5-40135) in ICC/IF
Immunofluorescence analysis of GLS was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Glutaminase C (GAC) Polyclonal Antibody (Product # PA5-40135) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Ce... View More
产品信息
PA5-40135
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the C-terminus region of human Glutaminase C.
偶联物
Unconjugated
形式
Liquid
浓度
0.96 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2609957
产品详细信息
This antibody specifically recognizes GAC but not the KGA isoform.
Predicted reactivity: Bovine (100%).
Recommended positive controls: MDA-MB-231 cells.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GLS; glutaminase C; Glutaminase kidney isoform, mitochondrial; glutaminase, phosphate-activated; K-glutaminase; L-glutamine amidohydrolase
基因别名: AAD20; GAC; GAM; GLS; GLS1; KGA; KIAA0838
UniProt ID: (Human) O94925
Entrez Gene ID: (Human) 2744
