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Invitrogen
Goat anti-Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 405
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Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody (A48260) in ICC/IF
Immunofluorescent analysis of β-III - tubulin in E18 Sprague Dawley dissociated rat cortical neuronal cells. 7 days post plating cells were fixed with 4% fromaldehyde for 20 mins, washed 3x in PBS, permeablized with 0.5% Triton X-100 in PBS for 20 minutes, washed 3x in PBS and blocked with 3% BSA in PBS for 30 minutes. Cells were incubated with chicken anti- β-III - tubulin diluted in 3% BSA in PBS overnigh... View More
产品信息
A48260
应用
建议稀释比
免疫细胞化学 (ICC/IF)
1-10 µg/mL
产品规格
种属反应
Chicken
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Purified Chicken IgY, Heavy and Light chains
偶联物
激发/发射光谱
404/455 nm
查看光谱
形式
Liquid
浓度
2 mg/mL
纯化类型
Affinity chromatography
保存液
proprietary buffer, pH 6.5
内含物
0.016% Methylisothiazolone, 0.016% Bromonitrodioxane
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2890271
靶标
IgY
交叉吸附
Against chicken serum containing non-immunoglobulin chicken serum proteins.
抗体形式
Whole Antibody
产品详细信息
To minimize cross-reactivity, the goat anti-chicken IgY whole antibodies have been affinity-purified and cross-adsorbed against chicken serum containing non-immunoglobulin chicken serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.
Specificity: This antibody binds to heavy chains on chicken IgY and light chains on all chicken immunoglobulins. This antibody does not bind non-immunoglobulin chicken serum proteins.
靶标信息
Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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