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Invitrogen
Goat anti-Human IgG (Heavy chain) Superclonal™ Secondary Antibody, HRP
高级验证
This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated.
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Human IgG (Heavy chain) Secondary Antibody (A56862) in WB
Western blot analysis was performed under reducing conditions on 30 µg whole-cell extracts of T-47D (Lane 1), HeLa (Lane 2), DU 145 (Lane 3), MCF-7 (Lane 4) and HCT 116 (Lane 5) using Novex® NuPAGE™ 3 to 8%, Tris-Acetate gel (Product # EA0375BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probe... View More
产品信息
A56862
应用
建议稀释比
免疫印迹 (WB)
1:10,000
酶联免疫吸附实验 (ELISA)
1:2,000-1:10,000
产品规格
种属反应
Human
宿主/亚型
Goat
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Secondary Antibody
抗原
Purified Human IgG
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with proprietary stabilizer
内含物
proprietary preservative
保存条件
4° C, do not freeze
运输条件
Wet ice
RRID
AB_2930927
靶标
IgG (Heavy chain)
抗体形式
Whole Antibody
产品详细信息
This Goat-anti-Human IgG (Heavy Chain), Superclonal Recombinant Secondary Antibody specifically detects human IgG Heavy chain with no cross-reactivity to light chain and other species immunoglobulins except moderate cross-reactivity towards Monkey IgG.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity, animal origin-free formulation, and lot-to-lot consistency.
Superclonal™ Recombinant antibodies are a defined pool of recombinant monoclonal antibodies, manufactured by selecting and pooling individual clones to provide complementary, specific, and optimal epitope coverage to achieve enhanced performance.
The sensitivity and specificity of each lot is confirmed using ELISA.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
