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Invitrogen
Goat anti-Human IgG (Kappa Light Chain) Recombinant Secondary Antibody, Alexa Fluor™ Plus 488
高级验证
This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated.
图: 1 / 5
Human IgG (Kappa Light Chain) Secondary Antibody (A56865) in ICC/IF
Immunofluorescence analysis was performed on fixed and permeabilized SK-BR-3 cells stained with HER2/Trastuzumab Recombinant Human Monoclonal Antibody (Product# MA5-42305, 1:100) and detected using Goat-anti-Human IgG (Kappa Light Chain), Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 (Product #A56865, 1:1,000). Panel a) shows representative image of cells stained for detection of HER2 receptor prote... View More
产品信息
A56865
应用
建议稀释比
免疫细胞化学 (ICC/IF)
1:500 - 1:1,000
流式细胞分析 (Flow)
1:1,000
产品规格
种属反应
Human
宿主/亚型
Goat
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Secondary Antibody
克隆号
81H59L2
抗原
Purified Human IgG
偶联物
激发/发射光谱
493/518 nm
查看光谱
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Affinity chromatography
保存液
proprietary buffer, pH 6.5
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4° C, store in dark
运输条件
Wet ice
RRID
AB_2937237
靶标
Kappa light chain
抗体形式
Whole Antibody
产品详细信息
This Goat-anti-Human IgG (Kappa Light Chain), Recombinant Secondary Antibody specifically detects human IgG Kappa Light chain with no cross-reactivity to Lambda light chain, heavy chain, and other species immunoglobulins.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
The sensitivity and specificity of each lot are confirmed using ELISA.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
