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Human IgM Secondary Antibody (31136) in ICC/IF
Immunofluorescence analysis of Goat anti-Human IgM Secondary Antibody was performed using log phaseRamos cells (IgM producing B-cell line). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with0.1% Triton™ X-100 for 15minutes, andblocked with 2% BSA for 1 hour at room temperature. The cells were labeled withGoat anti-Human IgM Secondary Antibody (Product # 31136) at 1:250 dilution... View More
产品信息
31136
应用
建议稀释比
免疫印迹 (WB)
1:2000
免疫组化 (IHC)
Assay-dependent
免疫细胞化学 (ICC/IF)
Assay-dependent
流式细胞分析 (Flow)
Assay-dependent
免疫沉淀 (IP)
Assay-dependent
产品规格
种属反应
Human
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
形式
Liquid
浓度
2.31 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.6
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_228279
靶标
IgM
抗体形式
Whole Antibody
产品详细信息
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31136 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31136 reacts with the Fc5µ portion of human IgM heavy chain. This antibody does not react against normal human IgG or IgA, or against non-immunoglobulin serum proteins. However, this antibody may cross-react with IgM from other species.
Store product at 4°C until opened. After opening, dilute only enough antibody for a single day's use. Store remainder at 4°C under sterile conditions.
Country of origin: USA
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
