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Mouse IgG F(ab')2 Secondary Antibody (31803) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 2 µg/mL) and detected by using Goat anti-Mouse IgG F (ab')2 Secondary Antibody, Biotin conjugate (Product # 31803) at dilutions 1:5,000 (Fig. 1), 1:50,000 (Fig. 2) and 1:100,000 (Fig. 3). A 18 kDa band correspondi... View More
产品信息
31803
应用
建议稀释比
免疫印迹 (WB)
1:20,000-1:400,000
免疫组化 (IHC)
1:500-1:5,000
免疫细胞化学 (ICC/IF)
1:500-1:5,000
流式细胞分析 (Flow)
1:200-1:1,000
酶联免疫吸附实验 (ELISA)
1:20,000-1:400,000
免疫沉淀 (IP)
1:500-1:5,000
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
形式
Lyophilized
浓度
1.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.6, with 15mg/mL BSA
内含物
0.05% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_228311
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31803 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31803 reacts with the light chains of mouse IgG and with those common to other mouse immunoglobulins. This antibody does not react with the Fc portion of mouse IgG or non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Rehydrate with 2 mL distilled water and centrifuge if not clear.
Country of Origin: USA
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
