Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin
图: 1 / 1
Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (62-6540) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin (Product # 62-6540) at dilutions 1:5,000 (Fig. 1) and 1:20,000 (Fig. 2). A 22 kDa band correspon... View More
产品信息
62-6540
应用
建议稀释比
免疫印迹 (WB)
1:5,000-1:20,000
免疫组化 (IHC)
1:8,000-1:10,000
酶联免疫吸附实验 (ELISA)
1:8,000-1:10,000
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobins Heavy and Light chains
偶联物
形式
Liquid
浓度
0.75 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 1% BSA, 40% glycerol
内含物
0.1% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2533949
靶标
IgG
交叉吸附
Against solid phase human serum protein
抗体形式
Whole Antibody
产品详细信息
ZyMAX antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
