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Mouse IgG (H+L) Secondary Antibody (31569) in ICC/IF
Immunofluorescence analysis of Goat anti-Mouse IgG (H+L) Secondary Antibody, FITC conjugate was performed using MCF-7 cells stained with Cytokeratin 19 (RCK108) Mouse Monoclonal Primary Antibody (Product # MA5-12613). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Mouse primary antibody (1:25... View More
产品信息
31569
应用
建议稀释比
免疫组化 (IHC)
1:50 - 1:200
免疫细胞化学 (ICC/IF)
1 µg/mL
免疫沉淀 (IP)
1:50 - 1:200
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Lyophilized
浓度
1.5 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.6, with 15mg/mL BSA
内含物
0.05% sodium azide
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_228306
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31569 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31569 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, based on electrophoresis. No antibody was detected against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: 9.6 µg/mg; > 3 moles FITC per mole IgG (lot-dependent).
Reconstitute with 1.5 mL of distilled water (1.5 mg/mL after restoration).
Country of Origin: USA
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
