Invitrogen

Goat anti-Mouse IgG (H+L) Secondary Antibody, PE

Mouse IgG (H+L) Secondary Antibody in Flow Cytometry (Flow)

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Mouse IgG (H+L) Secondary Antibody (M30004-1) in Flow

Flow cytometry analysis of Goat anti-Mouse IgG (H+L) Secondary Antibody, RPE (Product # M300041) was performed using K-562 cells stained with alpha Tubulin Mouse Monoclonal Antibody (Product # A11126). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with alpha Tubulin antib... View More

Mouse IgG (H+L) Secondary Antibody in Western Blot (WB)

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产品信息

M30004-1

应用

建议稀释比

免疫印迹 (WB)

1:2500

流式细胞分析 (Flow)

1:500

产品规格

种属反应

Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Secondary Antibody

抗原

Gamma Immunoglobins Heavy and Light chains

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with BSA

内含物

0.1% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2536617

靶标

IgG

交叉吸附

Against human immunoglobulins

抗体形式

Whole Antibody

靶标信息

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

仅用于科研。不用于诊断过程。未经明确授权不得转售。