Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ Plus 405
图: 1 / 2
Mouse IgG (H+L) Secondary Antibody (A55057) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor Plus 405 (Product # A55057; used at 4 µg/mL) was performed on fixed and permeabilized HeLa cells stained with mouse beta Tubulin Monoclonal Antibody (2 28 33) (Product # 32-2600, 1:500 dilution). Panel a) shows representative cells that were stained for detection and localiz... View More
产品信息
A55057
应用
建议稀释比
免疫细胞化学 (ICC/IF)
1-8 µg/mL
流式细胞分析 (Flow)
0.5-4 µg/mL
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Secondary Antibody
抗原
Purified Mouse Immunoglobulin
偶联物
激发/发射光谱
404/455 nm
查看光谱
形式
Liquid
浓度
2 mg/mL
纯化类型
Affinity chromatography
保存液
proprietary buffer, pH 6.5
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
AB_2921065
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity and superior lot-to-lot consistency.
Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig, and donkey IgG is observed.
Obtain superior images with the new Invitrogen Alexa Fluor Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor Plus secondary antibodies provide brighter signal compared to leading Alexa Fluor secondary antibodies, providing you with better signal-to-noise for your critical experiments. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning images.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
