Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 1
Mouse IgG, IgM (H+L) Secondary Antibody (A-10677) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG / IgM (H+L) Secondary Antibody, HRP conjugate (Product # A-10677) at dilutions 1:500 (Fig. 1), 1:1,000 (Fig. 2) and 1:2,000 (Fig. 3). A 22 kDa b... View More
产品信息
A-10677
应用
建议稀释比
免疫印迹 (WB)
1:500-1:2,000
免疫组化 (IHC)
1:500-1:2,000
酶联免疫吸附实验 (ELISA)
1:500-1:2,000
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Mouse IgG/IgM (H+L)
偶联物
形式
Lyophilized
纯化类型
Affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2534060
靶标
IgG/IgM
抗体形式
Whole Antibody
产品详细信息
Storage and reconstitution: The formulation of this antibody was updated from PBS with 0.02% thimerosal to PBS without thimerosal on 31/Mar/2024, any lots produced after this date will not contain thimerosal. Lot numbers greater than 2855945 will not have thimerosal.
Store product at -20°C until opened. After reconstitution in buffer without a stabilizer like thimerosal, the product is stable for approximately three months at 4°C. For prolonged storage after reconstitution in buffers without a stabilizer, add glycerol to a final concentration of 50% (v/v), aliquot, and store at -20°C. The antibody will be stable for up to one year stored in this manner.
Alternatively, if the product is reconstituted in a buffer containing a preservative, for example, in PBS, pH 7.2, containing 0.02% thimerosal, the product will be stable for up to one year when stored at 4°C.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
