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Invitrogen
F(ab')2-Goat anti-Mouse IgM (Mu chain) Secondary Antibody, Functional Grade, eBioscience™
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Mouse IgM (Mu chain) Secondary Antibody (16-5092-85) in Flow
Mouse splenocytes were unstimulated (left) or stimulated with 10 µg/mL of F (ab')2 Anti-Mouse IgM, u chain specific Functional Grade Purified (Product # 16-5092-85) for 48 hours (right) followed by staining with Anti-Mouse CD19 PE (Product # 12-0193-82) and CD86 APC (Product # 17-0862-82).Cells in the lymphocyte gate were used for analysis.
产品信息
16-5092-85
应用
建议稀释比
流式细胞分析 (Flow)
Assay-Dependent
功能检测 (Functional)
Assay-Dependent
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ Ig
分类
Polyclonal
类型
Secondary Antibody
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2573088
靶标
IgM
抗体形式
F(ab')2 Fragment
产品详细信息
Description: This polyclonal F(ab')2 fragment recognizes mouse IgM. Reactivity to human and bovine has been removed. This polyclonal antibody can be used to crosslink and activate B lymphocytes.
Applications Reported: This polyclonal F(ab')2 Goat Anti-Mouse IgM has been reported for use in functional assays (such as B cell activation). Concentrations should be determined empirically for optimal performance in the assay of interest.
Applications Tested: F(ab')2 Anti-Mouse IgM, u chain specific Functional Grade Purified has been tested by in vitro stimulation of mouse splenocytes.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.5 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
