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Invitrogen
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight™ 680
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (35569) in ICC/IF
Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 680 (Product # 35569) was performed using MCF 10A (positive model) and A-431 (negative model) cells stained with Vimentin Polyclonal Antibody (Product # PA5-27231). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 ho... View More
产品信息
35569
应用
建议稀释比
免疫印迹 (WB)
1:5,000-1:20,000
免疫组化 (IHC)
1:50-1:2,000
免疫细胞化学 (ICC/IF)
1:50-1:2,000
流式细胞分析 (Flow)
1:25 - 1:100
免疫沉淀 (IP)
Assay-dependent
产品规格
种属反应
Rabbit
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
激发/发射光谱
676/705 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2
内含物
0.02% sodium azide
保存条件
4° C
运输条件
Ambient
RRID
AB_1965957
靶标
IgG
交叉吸附
Against human, horse, mouse, swine, goat, bovine and rat serum proteins
抗体形式
Whole Antibody
产品详细信息
Product # 35569 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 35569 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. The antibody has been tested by solid-phase adsorbed to ensure minimal cross-reactivity with human, horse, mouse, swine, goat, bovine, and rat serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 680 Amax= 682 nm; Emax= 715 nm. Mole Dye/Mole Protein Ratio is lot-dependent.
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
