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Invitrogen
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3
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Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A10520) in WB
Multiplexed fluorescent western blot was performed using Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3 (Product # A10520). Whole cell extracts of THP-1 (Lane 1, 2, 3), MCF7 (Lane 4, 5), HeLa (Lane 6) and HEK-293 (Lane 7) were electrophoresed usingNuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP03222BOX). Resolved proteins were transferred onto anitrocellulose membrane (Product # IB2... View More
产品信息
A10520
应用
建议稀释比
免疫印迹 (WB)
1:10,000
免疫细胞化学 (ICC/IF)
1-10 µg/mL
产品规格
种属反应
Rabbit
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobins Heavy and Light chains
偶联物
激发/发射光谱
554/566 nm
查看光谱
形式
Liquid
浓度
2 mg/mL
纯化类型
purified
保存液
PBS, pH 7.5
内含物
5mM sodium azide
保存条件
4° C, store in dark
运输条件
Ambient
RRID
AB_10563288
靶标
IgG
交叉吸附
Against human IgG, human serum, mouse IgG and bovine serum
抗体形式
Whole Antibody
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
