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Rat IgG (H+L) Secondary Antibody (31350) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1) and F9 (Lane 2). The blots were probed with Anti-Alpha Tubulin Rat Monoclonal Antibody (Product # MA1-80017, 1 µg/mL) and detected by chemiluminescence for alkaline phosphatase substrate using Goat anti-Rat IgG (H+L) Secondary Antibody, AP (Product # 31350) at dilutions 1:10,000 (Fig. 1) and 1:50,000 (Fig. 2). A 52 kD... View More
产品信息
31350
应用
建议稀释比
免疫印迹 (WB)
1:10,000-1:50,000
产品规格
种属反应
Rat
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
形式
Lyophilized
浓度
0.6 mg/mL
纯化类型
Affinity chromatography
保存液
TBS, pH 8, with 15mg/mL BSA
内含物
0.05% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_228354
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
This antibody has been successfully used in Western blot and IP applications.
Antibody Specificity: This antibody reacts with the heavy chains of rat IgG and with the light chains common to most rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Restoration and Storage: Store product at 4°C until opened. Restore with 1.0 mL distilled water (0.6 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Country of Origin: USA
靶标信息
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
