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Rat IgG (H+L) Secondary Antibody (A18868) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of NCCIT (Lane 1) and F9 (Lane 2). The blots were probed with Anti-alpha Tubulin Rat Monoclonal Antibody (Product # MA1-80017, 1 µg/mL) and detected by chemiluminescence of alkaline phosphatase (AP) using Goat anti-Rat IgG (H+L) Secondary Antibody, AP conjugate (Product # A18868) at dilutions 1:500 (Fig. 1), 1:10,000 (Fig. 2) and 1:20... View More
产品信息
A18868
应用
建议稀释比
免疫印迹 (WB)
1:500-1:20,000
免疫组化 (IHC)
1:500-1:20,000
酶联免疫吸附实验 (ELISA)
1:500-1:20,000
产品规格
种属反应
Rat
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobins Heavy and Light chains
偶联物
形式
Liquid
浓度
1.5 mg/mL
纯化类型
Affinity chromatography
保存液
1% BSA, 30mM triethanolamine, 5mM MgCl2, 0.1mM ZnCl2
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2535645
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
靶标信息
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
