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The antibody has been shown to react specifically with rat IgG2a by immunoelectrophoresis and ELISA. The antibody may cross-react with IgG2a from other species.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Goat anti Rat IgG2a antibody recognizes rat IgG2a as assessed by immunoelectrophoresis and ELISA.Goat anti Rat IgG2a antibody may cross react with IgG2a from other species.
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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