Invitrogen

H-2Db Monoclonal Antibody (28-14-8), PE

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H-2Db Antibody (A15443) in Flow

Flow cytometry analysis of H-2Db in C57BL/6 mousesplenocytes. Cells were stained using H-2Db Monoclonal Antibody (28-14-8), PE (Product # A15443).

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产品信息

A15443

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/1x10^6 cells

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产品规格

种属反应

Mouse

已发表种属

Yeast

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

28-14-8

抗原

C3H.SW splenocytes (Mouse)

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偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.1 mg/mL

纯化类型

purified

保存液

PBS, pH 7.5

内含物

<0.1% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2534456

靶标信息

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

A Single-Domain TCR-like Antibody Selective for the Qa-1^b/Qdm Peptide Complex Enhances Tumoricidal Activity of NK Cells via Blocking the NKG2A Immune Checkpoint.

Journal of immunology (Baltimore, Md. : 1950)

Ghaffari S,Upchurch-Ange K,Gimlin S,Tripathi T,Sluijter M,Middelburg J,van Hall T,Weidanz J

A15443 was used in Flow Cytometry to show that EXX-1 has exquisite binding specificity for the Qa-1b/Qdm peptide complex, making it a valuable research tool for further investigation of the Qa-1b/Qdm peptide complex expression and regulation in healthy and diseased cells and for evaluation as an immune checkpoint blocking Ab in syngeneic mouse tumor models.

Sun May 01 00:00:00 EDT 2022

Microglia and Perivascular Macrophages Act as Antigen Presenting Cells to Promote CD8 T Cell Infiltration of the Brain.

Frontiers in immunology

Goddery EN,Fain CE,Lipovsky CG,Ayasoufi K,Yokanovich LT,Malo CS,Khadka RH,Tritz ZP,Jin F,Hansen MJ,Johnson AJ

Published figure using H-2Db monoclonal antibody (Product # A15443) in Flow Cytometry

Tue Jan 11 00:00:00 EST 2022

生物信息学

蛋白别名: H-2 class I histocompatibility antigen, D-B alpha chain; H-2D cell surface glycoprotein; H-2D(B); H2-DC1-beta transplantation antigen protein; H2D1; H2DB; H2L; H2LD; MHC class I antigen; MHC class I H-2Dp; MHC class I H2 antigen; MHC H2-D-q alpha-chain; Q5k protein

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基因别名: H-2D; H2-D; H2-D1

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UniProt ID: (Mouse) P01899

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Entrez Gene ID: (Mouse) 14964

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功能

receptor binding protein binding beta-2-microglobulin binding peptide binding peptide antigen binding T cell receptor binding poly(A) RNA binding TAP binding protein heterodimerization activity major histocompatibility complex protein

参与通路

positive regulation of T cell mediated cytotoxicity immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent immune response antigen processing and presentation