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H3.3 K27M oncohistone mutant Antibody (MA5-27916) in ICC/IF
Immunocytochemistry analysis of H3.3K27M on formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding Histone H3 K27M mutant, stained with H3.3 K27M oncohistone mutant Recombinant Monoclonal antibody (Product # MA5-27916).
产品信息
MA5-27916
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
RM192
抗原
A peptide corresponding to Histone H3 K27M mutant
偶联物
形式
Liquid
浓度
0.25 mg/mL
纯化类型
Protein A
保存液
PBS with 1% BSA, 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2744969
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Click here for Master Lot linking information.
靶标信息
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: H3 histone; H3 histone, family 3B (H3.3B); H3 K27; H3 K27M; H3/b; H3/d; H3/f; H3/j; H3a; H3K27 mutant; H3K27M; Histone 3; Histone H3 K27M; Histone H3.3; Histone H3K27M
基因别名: H3-3A; H3-3B; H3.3A; H3.3B; H3F3; H3F3A; H3F3B; PP781
UniProt ID: (Human) P84243
Entrez Gene ID: (Human) 3020, (Human) 3021
chromatin silencing at rDNA
nucleosome assembly
DNA replication-independent nucleosome assembly
brain development
blood coagulation
response to hormone
positive regulation of cell growth
gene silencing by RNA
telomere organization
cellular protein metabolic process
negative regulation of gene expression, epigenetic
positive regulation of gene expression, epigenetic
