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H3K4me2 Antibody (701764) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized MCF7 cells for detection of Histone H3K4me2 using Anti-Histone H3K4me2 Recombinant Rabbit Monoclonal Antibody (Product # 701764, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection and localization of Histone H3K4me2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant... View More
产品信息
701764
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Yeast
已发表种属
Plant
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
24H8L19
抗原
Methylated peptide (Lys4) corresponding to human Histone H3 (aa 4-10)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532505
产品详细信息
Since it is highly conserved across species, the antibody may react with many other species.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Di histone H3/c; di-methyl H3; di-methyl histone H3; Di-Methyl-H3 K4; Di-Methyl-H3 Lys4; Di-Methyl-Histone H3 K4; Di-Methyl-Histone H3 Lys4; dimethyl H3; Dimethyl Histone H3; H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K4me2; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3/b; H3/d; H3/f; H3/j; H3K4; H3Lys4me2; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; Histone 3; Histone H3; Histone H3.1; Histone H3.2; Histone H3.3; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K4; Histone H3K4me2; methyl Histone 3; methyl Histone H3
基因别名: BUR5; H3; H3-3A; H3-3B; H3.2; H3.3A; H3.3B; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3F1K; H3F2; H3F3; H3F3A; H3F3B; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; HHT1; HHT2; HIST1H3A; HIST1H3B; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; N2749; PP781; SIN2; YBR010W; YBR0201; YNL031C
UniProt ID: (Human) P84243, (Baker's yeast) P61830, (Human) P68431, (Human) Q71DI3
Entrez Gene ID: (Human) 3020, (Baker's yeast) 852295, (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
protein binding
nucleosomal DNA binding
histone binding
protein heterodimerization activity
DNA binding
cupric reductase activity
structural constituent of chromatin
cadherin binding involved in cell-cell adhesion
histone
chromatin silencing at rDNA
nucleosome assembly
DNA replication-independent nucleosome assembly
brain development
blood coagulation
response to hormone
positive regulation of cell growth
gene silencing by RNA
telomere organization
cellular protein metabolic process
negative regulation of gene expression, epigenetic
positive regulation of gene expression, epigenetic
chromatin organization
cellular copper ion homeostasis
aerobic respiration
rRNA transcription
sexual sporulation resulting in formation of a cellular spore
global genome nucleotide-excision repair
positive regulation of defense response to virus by host
DNA replication-dependent nucleosome assembly
protein heterotetramerization
regulation of gene silencing
cell-cell adhesion
xenophagy
