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Invitrogen
H3K4me3 Polyclonal Antibody
高级验证
This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated.
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H3K4me3 Antibody (49-1005) in ICC/IF
Immunofluorescence analysis of Tri-Methyl-Histone H3 (Lys4) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rabbit Polyclonal Antibody (Product # 49-1005) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More
产品信息
49-1005
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Raised against the region of the histone H3 containing the trimethylated lysine 4 (or [K4me3]), using a KLH-conjugated synthetic peptide.
偶联物
形式
Liquid
浓度
1.3 mg/mL
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Dry ice
RRID
AB_2533856
产品详细信息
49-1005, H3K4me3 antibody, has been shown to recognize several plant species: Arabidopsis, rice, tomato, maize, poplar, and silena latifolia.
靶标信息
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
