Invitrogen

HDGF Recombinant Rabbit Monoclonal Antibody (JE56-32)

HDGF Antibody in Immunocytochemistry (ICC/IF)

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HDGF Antibody (MA5-42522) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of of HDGF in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HDGF Recombinant Monoclonal Antibody (Product # MA5-42522) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

HDGF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-42522

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:100

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE56-32

抗原

Recombinant protein within human HDGF aa 50-200

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2911663

产品详细信息

Positive Control: Rat cerebellum tissue lysate, SK-Br-3 cell lysate, A549 cell lysate, mouse cerebellum tissue lysate, F9, human liver carcinoma tissue, human cervix tissue, human colon carcinoma tissue, human breast carcinoma tissue, human esophagus tissue, human stomach carcinoma tissue, human small intestine tissue, THP-1.

Subcellular Location: Nucleus, Cytoplasm, Extracellular exosome.

靶标信息

HDGF is a member of the hepatoma-derived growth factor family. This protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。