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Invitrogen
HELIOS Monoclonal Antibody (22F6), PerCP-eFluor™ 710, eBioscience™
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HELIOS Antibody (46-9883-42) in Flow
Intracellular staining of BALB/c lymph node cells with Anti-Mouse/Rat Foxp3 eFluor® 450 (Product # 48-5773-82) and Armenian Hamster IgG Isotype Control PerCP-eFluor® 710 (Product # 46-4888-82) (left) or Anti-Helios PerCP-eFluor® 710 (right) using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis.
产品信息
46-9883-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Human,
Mouse
已发表种属
Mouse
宿主/亚型
Armenian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
22F6
偶联物
激发/发射光谱
482/708 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2573924
产品详细信息
Description: This 22F6 monoclonal antibody reacts with human, mouse and canine Helios. Helios is a zinc-finger transcription factor first identified for its similarity to Ikaros. It is expressed in hematopoietic stem cells in both the embryo and adult mice. While the highest expression is found in regulatory T cells, Helios is not expressed in mature B cells, dendritic cells or myeloid cells. Helios can form a homodimer as well as heterodimerize with other Ikaros family members to regulate transcription. Eight isoforms of Helios exist, some of which possess a dominant-negative function that has also been found to be upregulated in lymphoid malignancies, including T-acute lymphoblastic leukemia.
22F6 has been reported to cross react with rat Helios.
Applications Reported: This 22F6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 22F6 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse lymph node cells using the using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.00375 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: IKAROS family zinc finger 2 (Helios); Ikaros family zinc finger protein 2; zinc finger DNA binding protein Helios; Zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios)
基因别名: A730095J18Rik; ANF1A2; HELIOS; IKZF2; Zfpn1a2; ZNF1A2; ZNFN1A2
UniProt ID: (Human) Q9UKS7, (Mouse) P81183
Entrez Gene ID: (Dog) 488510, (Human) 22807, (Mouse) 22779
