OriGene

HES1 Monoclonal Antibody (OTI2D2), TrueMAB™

查看其他15个HES1抗体

抗体检测数据 (8)

HES1 Antibody in Immunocytochemistry (ICC/IF)

HES1 Antibody in Immunocytochemistry (ICC/IF)

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HES1 Antibody (CF504001) in ICC/IF

Anti-HES1 mouse monoclonal antibody (TA504001) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HES1(RC211709).

HES1 Antibody in Immunocytochemistry (ICC/IF)

HES1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HES1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HES1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HES1 Antibody in Western Blot (WB)

HES1 Antibody in Western Blot (WB)

HES1 Antibody in Western Blot (WB)

HES1 Antibody in Flow Cytometry (Flow)

Product Image Gallery

HES1 Antibody in Immunocytochemistry (ICC/IF) — Anti-HES1 mouse monoclonal antibody (TA504001) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HES1(RC211709).

HES1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human thyroid tissue within the normal limits using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504001)

HES1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504001)

HES1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504001)

HES1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (RC211709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1. Positive lysates LY417251 (100 µg) and LC417251 (20 µg) can be purchased separately from OriGene.

HES1 Antibody in Western Blot (WB) — Western blot analysis of extracts (10 µg) from a mouse cell line and 3 different mouse tissues by using anti-HES1 monoclonal antibody. (1:200)

HES1 Antibody in Western Blot (WB) — Western blot analysis of extracts (10 µg) from 8 different cell lines by using anti-HES1 monoclonal antibody. (1:200)

HES1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Jurkat cells, using anti-HES1 antibody (TA504001), (Red), compared to a nonspecific negative control antibody, (Blue).

产品信息

CF504001

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

-

免疫组化（石蜡） (IHC (P))

1:150

-

免疫细胞化学 (ICC/IF)

1:100

-

流式细胞分析 (Flow)

1:100

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OTI2D2

抗原

Full length human recombinant protein of human HES1 produced in HEK293T cell.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 8% trehalose

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

靶标信息

This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: bHLHb39; Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1; Hairy homolog; Hairy-like; Hairy-like protein; hHL; Transcription factor HES-1

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基因别名: BHLHB39; HES-1; HES1; HHL; HL; HRY

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UniProt ID: (Human) Q14469

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Entrez Gene ID: (Human) 3280

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transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding chaperone binding N-box binding basic helix-loop-helix transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter liver development embryonic heart tube morphogenesis outflow tract morphogenesis regulation of secondary heart field cardioblast proliferation ventricular septum development transcription, DNA-templated protein complex assembly cell adhesion Notch signaling pathway smoothened signaling pathway STAT protein import into nucleus nervous system development positive regulation of cell proliferation cell migration telencephalon development midbrain-hindbrain boundary morphogenesis oculomotor nerve development trochlear nerve development hindbrain morphogenesis forebrain radial glial cell differentiation adenohypophysis development lung development positive regulation of BMP signaling pathway midbrain development pancreas development somatic stem cell population maintenance ascending aorta morphogenesis positive regulation of T cell proliferation positive regulation of tyrosine phosphorylation of Stat3 protein auditory receptor cell fate determination positive regulation of DNA binding regulation of fat cell differentiation negative regulation of auditory receptor cell differentiation positive regulation of Notch signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of mitotic cell cycle, embryonic lateral inhibition positive regulation of JAK-STAT cascade cell maturation thymus development cell morphogenesis involved in neuron differentiation positive regulation of astrocyte differentiation negative regulation of oligodendrocyte differentiation artery morphogenesis regulation of epithelial cell proliferation inner ear receptor stereocilium organization regulation of timing of neuron differentiation negative regulation of glial cell proliferation ventricular septum morphogenesis ureteric bud morphogenesis labyrinthine layer blood vessel development common bile duct development negative regulation of stomach neuroendocrine cell differentiation cardiac neural crest cell development involved in outflow tract morphogenesis pharyngeal arch artery morphogenesis glomerulus vasculature development comma-shaped body morphogenesis S-shaped body morphogenesis renal interstitial fibroblast development metanephric nephron tubule morphogenesis cochlea development establishment of epithelial cell polarity vascular smooth muscle cell development neuronal stem cell population maintenance positive regulation of protein targeting to mitochondrion negative regulation of pancreatic A cell differentiation negative regulation of stem cell differentiation negative regulation of pro-B cell differentiation negative regulation of forebrain neuron differentiation