Invitrogen

HIP1 Polyclonal Antibody

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HIP1 Antibody (PA5-93288) in WB

Western blot analysis of HIP1 in extracts of various cell lines. Samples were incubated with HIP1 Polyclonal antibody (Product # PA5-93288) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

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产品信息

PA5-93288

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 410-690 of human HIP1 (NP_0053293)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.43 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2806705

产品详细信息

Immunogen sequence: SELEADLAEQ QHLRQQAADD CEFLRAELDE LRRQREDTEK AQRSLSEIER KAQANEQRYS KLKEKYSELV QNHADLLRKN AEVTKQVSMA RQAQVDLERE KKELEDSLER ISDQGQRKTQ EQLEVLESLK QELATSQREL QVLQGSLETS AQSEANWAAE FAELEKERDS LVSGAAHREE ELSALRKELQ DTQLKLASTE ESMCQLAKDQ RKMLLVGSRK AAEQVIQDAL NQLEEPPLIS CAGSADHLLS TVTSISSCIE QLEKSWSQYL ACPEDISGLL H; Positive Samples: A-549, BT-474, SW480, 293T, Mouse brain; Cellular Location: Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Nucleus, clathrin-coated vesicle membrane

靶标信息

Huntingtin disease, a neurodegenerate disease, is caused by the expansion of a polymorphic glutamine tract in huntingtin. The Huntingtin Interacting Protein 1 (HIP-1) is a reportedly proapoptotic, cargo-specific adaptor protein that may be involved in the pathogenesis of Huntingtin disease. As well as playing a role in Huntingtin disease, it is likely to be involved in the recruitment of clathrin coats to lipid membranes and it may also factor in tumorigenesis by allowing the survival of precancerous and cancerous cells. Since HIP-1 expression is significantly associated with prostate and colon cancer metastasis, HIP-1 can serve as a putative prognostic factor for prostate and colon cancers.

仅用于科研。不用于诊断过程。未经明确授权不得转售。