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HIV1 p55 Antibody (PA1-4959) in WB
Detection of HIV-1 p55 by Western blotting using an HIV1 p55 Polyclonal Antibody (Product # PA1-4959). Lane1: Extract of MT4 cells. Lane2: Extract of MT4 cells infected with HIV-1 (LAI strain). This antibody reacts with not only p55 but also the processed products p24 and p17. The other bands are intermediates of processing. The antiserum was diluted 1:2,000.
产品信息
PA1-4959
应用
建议稀释比
已发表文章
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Purified full-size recombinant Gag p55 of HIV1 subtype B expressed in E. coli.
偶联物
Unconjugated
形式
Liquid
浓度
Conc. Not Determined
保存条件
-20°C
运输条件
Wet ice
RRID
AB_612297
产品详细信息
PA1-4959 detects the HIV-1 p55 protein in HIV samples.
PA1-4959 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~55 kDa protein, corresponding to HIV-1 p55 in HIV samples. Also detected are a ~15 kDa, ~17 kDa and ~24 kDa protein, corresponding to the digested proteins.
PA1-4959 immunogen corresponds to the recombinant p55 protein which was over-expressed in E. coli with a plasmid carrying the Gag p55 coding region of HIV-1 virus.
Recombinant HIV-1 Gag p55 protein (Cat. # RP-4920 or RP-4921) can be purchased for control experiments in Western blot assays.
靶标信息
HIV-1 Gag p55 is a precursor protein of several proteins that form the core structure of AIDS virus, indispensable to its reproduction. This protein is digested by HIV-1 protease, first into intermediate products p41 and p15. Then p41 is digested into matrix protein p17 and capsid protein p24. Protein p15 is further digested into nucleocapsid protein p7 and to p6 and p1, both of unknown function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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