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HK2 Antibody (PA5-102329) in ICC/IF
Immunofluorescent analysis of HK2 in HEPG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% saponin, blocked with 10% serum (45 min at 25°C) incubated with HK2 polyclonal antibody (Product # PA5-102329) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) at a dilution of 1:600.
产品信息
PA5-102329
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human HK2(Accession P52789), corresponding to amino acid residues P884-R917.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2851737
产品详细信息
Antibody detects endogenous levels of total Hexokinase II.
靶标信息
In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp686M1669; Hexokinase; Hexokinase type II; Hexokinase-2; hexokinase-2 muscle; hexokinase-2, muscle; Hexokinase-B; HK II; Muscle form hexokinase; OTTHUMP00000202738
基因别名: AI642394; HK2; HKII; HXK2
UniProt ID: (Human) P52789, (Mouse) O08528, (Rat) P27881
Entrez Gene ID: (Human) 3099, (Mouse) 15277, (Rat) 25059
cellular glucose homeostasis
response to ischemia
glycolytic process
lactation
apoptotic mitochondrial changes
glucose transport
negative regulation of mitochondrial membrane permeability
regulation of glucose import
carbohydrate phosphorylation
glucose 6-phosphate metabolic process
canonical glycolysis
establishment of protein localization to mitochondrion
maintenance of protein location in mitochondrion
positive regulation of mitophagy in response to mitochondrial depolarization
negative regulation of reactive oxygen species metabolic process
carbohydrate metabolic process
glucose metabolic process
metabolic process
phosphorylation
glucose catabolic process
